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β(1-3,4,6)-galactosidase [For Glycan Release] (CAT#: CB-P266-K) Datasheet

Product Type
Kit
Description
Β(1-3,4,6) galactosidase cleaves all non-reducing terminal galactoses linked to β(1-3) and β(1-4). β(1-6) linked galactose The sugar is released at a slower rate. The enzyme is a glycoprotein.
So far, β(1-4) galactose is the most common linkage among N-linked oligosaccharides. For the cleavage of this connection, we recommend the use of β(1-4) galactosidase (CB-P265-K catalog).
Features
Source: Bovine Testes

EC: 3.2.1.23

Alternate Names: β-D-galactoside galactohydrolase, Exo-(1-4)-β-D-galactanase, Lactase, β(1-3,4,6) galactosidase

Specific Activity:>10.2 U/mg
Activity:>2.5 U/mL

Molecular weight: 68,000 daltons

Rxn pH: 4

Specificity: Cleaves all β(1-3) and β(1-4) linked non-reducing, terminal galactose.

Specific Activity: One unit of β(1-3,4,6) Galactosidase is defined as the amount of enzyme required to produce 1 µmole of p-nitrophenol (pNP) in 1 minute at 37°C, pH 4.0 from p-nitrophenyl-β-D-galactopyranoside.
Applications
Cleaves all β(1-3) and β(1-4) linked non-reducing, terminal galactose. β(1-6) linked galactose is released at a slower rate.
Size
0.5 U / 200 µL; Sufficient for up to 200 reactions.
Sample
Glycoproteins and glycopeptides containing N-linked glycans
Components
200 µL of β(1-3,4,6) Galactosidase in 20 mM Tris-HCl, 50 mM NaCl, 0.5mg/mL BSA, pH 7.5
5x Reaction Buffer- 500 mM sodium citrate/phosphate pH 4
Handling Advice
1. Add up to 100 µg of asialoglycoprotein or 1 nmol of oligosaccharide to tube.
2. Add deionized water to 14 µL.
3. Add 4 µL 5x Reaction Buffer 4.0.
4. Add 2 µL of enzyme
5. Incubate one hour at 37°C.
Storage
Storage: Store enzyme at -20°C.
Shelf Life
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Research Use
For research use only. Not for human or drug use

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All products and services are for Research Use Only. Do Not use in humans.

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