β(1-3,4,6)-galactosidase [For Glycan Release] (CAT#: CB-P266-K) Datasheet

Product Type
Kit
Description
Β(1-3,4,6) galactosidase cleaves all non-reducing terminal galactoses linked to β(1-3) and β(1-4). β(1-6) linked galactose The sugar is released at a slower rate. The enzyme is a glycoprotein.
So far, β(1-4) galactose is the most common linkage among N-linked oligosaccharides. For the cleavage of this connection, we recommend the use of β(1-4) galactosidase (CB-P265-K catalog).
Features
Source: Bovine Testes

EC: 3.2.1.23

Alternate Names: β-D-galactoside galactohydrolase, Exo-(1-4)-β-D-galactanase, Lactase, β(1-3,4,6) galactosidase

Specific Activity:>10.2 U/mg
Activity:>2.5 U/mL

Molecular weight: 68,000 daltons

Rxn pH: 4

Specificity: Cleaves all β(1-3) and β(1-4) linked non-reducing, terminal galactose.

Specific Activity: One unit of β(1-3,4,6) Galactosidase is defined as the amount of enzyme required to produce 1 µmole of p-nitrophenol (pNP) in 1 minute at 37°C, pH 4.0 from p-nitrophenyl-β-D-galactopyranoside.
Applications
Cleaves all β(1-3) and β(1-4) linked non-reducing, terminal galactose. β(1-6) linked galactose is released at a slower rate.
Size
0.5 U / 200 µL; Sufficient for up to 200 reactions.
Sample
Glycoproteins and glycopeptides containing N-linked glycans
Components
200 µL of β(1-3,4,6) Galactosidase in 20 mM Tris-HCl, 50 mM NaCl, 0.5mg/mL BSA, pH 7.5
5x Reaction Buffer- 500 mM sodium citrate/phosphate pH 4
Handling Advice
1. Add up to 100 µg of asialoglycoprotein or 1 nmol of oligosaccharide to tube.
2. Add deionized water to 14 µL.
3. Add 4 µL 5x Reaction Buffer 4.0.
4. Add 2 µL of enzyme
5. Incubate one hour at 37°C.
Storage
Storage: Store enzyme at -20°C.
Shelf Life
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Research Use
For research use only. Not for human or drug use

All products and services are for Research Use Only. Do Not use in humans.

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