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Hexokinase Activity Assay Kit (Fluorometric) (CAT#: CB-P167-K) Datasheet

Product Type
Kit
Description
Hexokinase activity assay kit (Fluorometric) provides a simple, sensitive and rapid to monitor the activity of hexokinase (HK) in cells, serum, and animal and plant tissues. In this assay, HK converts glucose into glucose 6-phosphate, and then glucose undergoes a series of reactions and reduces the sensitive probe to generate a strong fluorescent product, which can be easily detected at Ex / Em = 535/587 nm.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Sensitivity
2 µU
Sample
Cell Lysate
Species Reactivity
Mammals
Protocol Length
0h 20m
Components
ATP (50 µmol) 1 vial
HK Assay Buffer 1 x 25ml
HK Developer (50 U) 1 vial
HK Enzyme (100 U) 1 vial
HK Positive Control (500 mU) 1 vial
HK Substrate 1 x 1ml
NADPH Standard (200 nmol) 1 vial
PicoProbe (in DMSO) 1 x 400µl
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Typical NADPH standard calibration curve.


Figure 2 Kinetic curves showing Hexokinase activity in positive control (included in kit), lysates from HeLa (0.62 µg), Jurkat (0.65 µg) and MCF-7 cells (0.56 µg), and lysates from orchid leaf (4 µg).


Figure 3 Hexokinase specific activity in lysates from orchid leaf (4 µg), MCF-7 (0.56 µg), Jurkat (0.65 µg) and HeLa cells (0.62 µg).

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All products and services are for Research Use Only. Do Not use in humans.

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