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Antibody IgG N-glycosylation kit, Complete (CAT#: CB-P193-K) Datasheet

Product Type
The complete kit also contains isotopically labeled three most common α-2,3-sialylated glycan standards. Although they are naturally present in small amounts in the Fc portion of antibodies, they can strongly affect effector functions.
The kits, are low- and high-throughput glycan analysis kits, based on highly pure synthetic isotope-labeled internal standards for the absolute quantification of mAb glycosylation by MALDI-TOF MS. Minimal handling and time are required for sample preparation and within seconds, all relevant glycans of a deglycosylated mAb sample are quantified by comparison of MALDI profiles with the corresponding internal reference standards.
The glycan analysis is based on the isotopic dilution method and applied to the absolute determination of N-glycans in monoclonal antibodies by MALDI-TOF MS. Isotope dilution analysis is a method of internal standardization, based on the dilution of a mixture of analytes with their isotopically-enriched equivalents, allowing analysis by means of signal ratios. Isotopic dilution is considered a reference method for the absolute quantification of analytes in a mixture.
1 kit
Detection Method
Cell culture supernatant, Urine, Serum, Plasma, Tissue, Adherent cells, Suspension cells, Tissue Homogenate
Species Reactivity
Protocol Length
1h 00m
Filter device (10 x Amicon 30K spin filter)
Component A. PNGase F (lyophilized)
Component B. standards (Complete Kit 1.6, 100 µL)
Component C. Stabilization reagent (10 mg)
Component D. Sodium citrate tribasic (1 mM solution, 1 mL)
Component E. MALDI-TOF Matrix (DHB, 2 mg)
SDS (2% solution)
Ultra pure water (15 mL for mAb desalting)
USB flash drive. Glycan Quantification Software
Two empty bottles, labeled as HPLC grade Acetonitrile and HPLC
grade Ethanol to be filled by the user.
Table-top shaker with heating unit.
Centrifuge suitable for 2 mL Eppendorf tubes (floor or table-top).
Centrifugal evaporator suitable for 2 mL Eppendorf tubes.
HPLC grade acetonitrile (1 mL). Do not use lower purity.
Acetic acid (1 mL).
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 MALDI spectra of the labeled standards.

All products and services are for Research Use Only. Do Not use in humans.


Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

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