sCD59 ELISA Kit [Human], 2 x 96 det. (CAT#: CB-P136-K) Datasheet

Product Type
Kit
Description
CD59 is a cell surface glycoprotein that can regulate complement-mediated cell lysis and participate in lymphocyte signal transduction. Complement activation eventually leads to the formation of cytotoxic pores forming membrane attack complex (MAC), the main effector of complement-mediated tissue damage. The insertion of MAC into the cell membrane induces the release of cytokines and growth factors, thereby promoting inflammation, thrombosis and cell proliferation. CD59 is a complement regulatory protein ubiquitously expressed on the surface of mammalian cells, which specifically inhibits the formation of MAC, thereby protecting "self" cells from complement-mediated damage. The protein also plays a role in the signal transduction pathway of T cell activation. Mutations in the CD59 gene can cause CD59 deficiency, which can lead to hemolytic anemia and thrombosis, and lead to cerebral infarction. It turns out that the content of CD59 in the plasma/serum of healthy individuals ranges from 22 to 119 ng/ml.
Applications
The sCD59 ELISA kit is used for the in vitro quantitative determination of human sCD59 in plasma.
Principle
sCD59 ELISA kit is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle. The working time is 3 hours. The effective form of the plate with twelve disposable 8-well plates allows free choice of batch size for determination. The samples and standards are incubated in microtiter wells coated with antibodies that recognize human sCD59. The biotinylated tracer antibody will bind to the captured human sCD59. The streptavidin-peroxidase conjugate will bind the biotinylated tracer antibody. The streptavidin-peroxidase conjugate will react with the substrate tetramethylbenzidine (TMB). The enzyme reaction is stopped by adding oxalic acid. Measure the absorbance at 450nm with a spectrophotometer. The standard curve was obtained by plotting the absorbance (linear) against the corresponding concentration (logarithm) of the human sCD59 standard. The concentration of the human sCD59 sample running at the same time as the standard can be determined from the standard curve.
Size
2 x 96 det.
Detection Method
Enzyme immunoassay (ELISA) technique
Detection Limit
Measurable concentration range of 0.39 to 25 ng/ml.
Sensitivity
Minimum concentration which can be measured is 0.39 ng/ml.
Sample
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Specimen Collection
Blood samples were collected using sterile venipuncture techniques, and serum was obtained using standard procedures. At least 5 ml of whole blood is recommended. Let the blood clot in the serum test tube for 60-65 minutes at room temperature (20-25°C). Centrifuge the blood sample and transfer the cell-free serum to a clean test tube. The serum must be handled correctly to prevent complement activation in vitro. Serum should be frozen in a sealed tube at -70°C or lower for long-term storage or transport on dry ice. The sample should not be frozen and thawed more than once. Do not use jaundice, lipemia and hemolytic serum. You cannot use heat-inactivated serum. Plasma cannot be used.
Sample Volume
100 µl/well
Species Reactivity
Human
Cross Reactivity
Mouse - No, Pig - No, Rat - No
Protocol Length
2 hours
Storage
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.
Research Use
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.

All products and services are for Research Use Only. Do Not use in humans.

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