α(1,6) Core Mannosidase [For Glycan Release] (CAT#: CB-P270-K) Datasheet

Kit

Core alpha mannosidase cleaves the unbranched non-reducing terminal mannose, and the β-linked core mannose linked to the core of the conservative N-linked oligosaccharide mannosyl chitobiose (1-6). With the core The fucose linked to N-acetylglucosamine does not have an effect on cleavage.

If there is uncleavable α(1-6)mannose on the substrate, the enzyme may inhibit other mannosidases, such as α(1-2,3,6)mannosidase (CB-P269-K catalog). Therefore, it should always be added after digestion with other mannosidases.

Source: recombinant from Xanthamonas manihotis E. Coli

EC: 3.2.1.24

Alternate Names: α-D-mannosidase, p-nitrophenyl-α-mannosidase, α-D-mannopyranosidase, core α mannosidase, exo-α-mannosidase

Formulation:
The enzyme is provided as a sterile solution in 50 mM sodium phosphate pH 7.5, 0.1 mM ZnCl2.

Specific Activity:>1.5 U/mg
Activity: 1 U/mL

Molecular weight: ~52,000 daltons

Specific Activity Assay: One unit of α(1-6) Mannosidase is defined as the amount of enzyme required to produce 2 µmoles of mannose in 1 minute at 37°C, pH 5.0 from α(1-6) mannobiose.

α(1-6) Core Mannosidase cleaves unbranched non-reducing terminal mannose, α(1-6) linked to the β-linked core mannose of the conserved mannosylchitobiose core of N-linked oligosaccharides.