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Galactose and Lactose Assay Kit (Colorimetric/Fluorometric) (CAT#: CB-P188-K) Datasheet

Product Type
Kit
Description
Creative Biolabs' galactose and lactose content assay kits provide a simple for directly measuring the galactose and lactose content in various biological samples (serum, plasma, other body fluids, food, growth medium, etc.). In order to detect galactose, galactose oxidase specifically oxidizes free galactose to produce a product that reacts with the galactose probe to produce color (OD570nm) and Fluorometric (Ex / Em 535/587). To detect lactose, lactose is hydrolyzed by lactase to produce free galactose, which is then measured. Therefore, lactose level = total galactose-free galactose.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Sample
Cell culture supernatant, Urine, Serum, Plasma, Tissue, Adherent cells, Suspension cells, Tissue Homogenate
Species Reactivity
Mammals
Protocol Length
1h 00m
Components
Galactose Assay Buffer 1 x 25ml
Galactose Enzyme Mix (Lyophilized) 1 vial
Galactose Probe (DMSO solution) 1 x 200µl
Galactose Standard (100nmol/ul) 1 x 100µl
HRP (Lyophilized) 1 vial
Lactase (Lyophilized) 1 vial
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Example of a Colorimetric standard curve.

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All products and services are for Research Use Only. Do Not use in humans.

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