Lectin Complement Pathway ELISA Kit [Mouse], 2 x 96 det. (CAT#: CB-P126-K) Datasheet

Kit

This kit can measure the activity of the lectin pathway mediated by MBL. It can be easily screened for complement defects or other defects in the complement system by analyzing each pathway in parallel or separately. ELISA includes a positive control that can be used as a process control To ensure that the lectin complement cascade is fully performed, this positive control cannot be used to determine the activation level between batches. We recommend that you include a reference sample of 100% complement activity and a negative control (for example, unsaved) activation in the study Serum) to determine the activation level of the sample. Please note that the level of complement activity depends on the strain and may be affected by the way the sample is collected and processed.
The innate immune system is important in the important defense against foreign pathogens. The main component of this response is the complement system. The complement system consists of a complex family of proteins and receptors, which are found in the circulatory system, tissues and other body fluids. Today, some connections with adaptive immunity are also described. The system consists of three defined pathways, which are activated by pathway-specific molecules. Complement activation proceeds in a sequential manner through the proteolytic cleavage of a series of proteins, resulting in the production of activation products that mediate various biological activities through interactions with specific cell receptors and other serum proteins. These three pathways are the classical pathway, the lectin pathway and the alternative pathway, which converge into the final universal pathway at the central component. That is, the activation of C3 leads to the formation of C3a and C3b. This cleavage activates the terminal complement pathway, which ultimately leads to the formation of the terminal C5b-9 complement complex (TCC). The classical pathway is initiated by the binding of C1q to the antibody complex, while the alternative pathway and the lectin pathway interact with specific carbohydrate groups and lipopolysaccharide (LPS) on the surface of the foreign pathogen through complement components, and the antibody The independent way is activated. Alternative pathways also serve as amplification loops for other pathways.
Under certain conditions, the complement system may be detrimental to the host, causing for example. Autoimmune diseases and infections. The lack of C3 is, for example, related to SLE. Changes in alternative pathways, such as properdin or fibronectin deficiency, can increase susceptibility to infection. Mannose-binding lectin (MBL) is the main component of the lectin pathway and is related to bacterial, fungal and viral infections. A common method for measuring the activity of the classical or alternative pathway is hemolysis of red blood cells. In addition, several assays have been described to measure the activity of the MBL pathway. Not all determinations are easy to perform. By using a combination of specialized coatings and buffers, ELISA for the mouse route is easy to use and specific for each route. Complement defects or other defects in the complement system can be easily screened by analyzing each pathway in parallel or separately.

The mouse lectin complement pathway ELISA is used to determine the activation of the lectin pathway of the complement system in serum samples in vitro.

Mouse lectin complement ELISA kit is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle. The working time is 3 hours. The effective form of the plate with twelve disposable 8-well plates allows free choice of batch size for determination. Samples and positive controls are incubated in microtiter wells coated with antibodies. The biotinylated tracer antibody will bind to the bound C5b-9 in the sample and control. The streptavidin-peroxidase conjugate will bind the biotinylated tracer antibody. Streptavidin peroxidase will react with the substrate tetramethylbenzidine (TMB). The enzyme reaction is stopped by adding oxalic acid. Measure the absorbance at 450nm with a spectrophotometer.