Product Type
Kit
Description
In Creative Biolabs' lactose assay kit, lactose is hydrolyzed into glucose and galactose. Galactose is then oxidized to produce color (OD570nm) and Fluorometric (Ex / Em 535/587 nm). Free galactose can be corrected by background control in the absence of lactase. The lactose assay kit provides a simple, convenient and sensitive for directly measuring the lactose level in various biological samples (serum, plasma, other body fluids, food, growth medium, etc.). No sample pretreatment is required. The kit can be used for high-throughput assay.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Sample
Cell culture supernatant, Urine, Serum, Plasma, Tissue, Adherent cells, Suspension cells, Tissue Homogenate
Protocol Length
1h 00m
Components
HRP (Lyophilized) 1 vial
Lactase (Lyophilized) 1 vial
Lactose Assay Buffer 1 x 25ml
Lactose Enzyme Mix (Lyophilized) 1 vial
Lactose Standard (100 nmol/ul) 1 x 100µl
Probe (DMSO solution) 1 x 200µl
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use
