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L-Lactate Assay Kit (Colorimetric/Fluorometric) (CAT#: CB-P141-K) Datasheet

Product Type
Kit
Description
The L-lactic acid assay kit (colorimetric/Fluorometric) provides a convenient to detect L(+)-lactic acid in biological samples such as blood, cells, culture media, and fermentation media. Treatment or purification.
In the lactate assay protocol, lactate reacts specifically with the enzyme mixture to produce a product, which interacts with the lactate probe to produce color (570 nm) and Fluorometric (at Ex / Em = 535/587 nm).
The amylase assay kit (colorimetric) detects the activity of α-amylase through a two-step reaction.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Detection Limit
0.001 mM - 10 mM
Sensitivity
> 0.001 mM
Sample
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate
Species Reactivity
Mammals
Protocol Length
0h 40m
Components
L(+)-Lactate Standard (100 nmol/µl) 1 x 100µl
Lactate Assay Buffer1 x 25ml
Lactate Enzyme Mix (lyophilized)1 vial
Lactate Probe (in DMSO, anhydrous)1 x 200µl
Requires
Microplate reader
Handling Advice
Lactate assay protocol summary:
- add samples (deproteinized) and standards to wells
- add reaction mix and incubate for 30 min at room temp
- analyze with microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Relative signal (RFU) in unfiltered human plasma (dilution 1:200) and mouse urine (dilution 1:200), comparing L-lactate signals with background readings ((-); no enzyme) after 10 minutes of incubation (duplicates +/- SD).


Figure 2 Standard curve: mean of duplicates (+/- SD) with background reads subtracted.


Figure 3 Standard curve: mean of duplicates (+/- SD) with background reads subtracted.


Figure 4 Lactate measured in cell culture supernatants showing quantity (µmol) per mL of tested sample. Samples were diluted 40-160 fold and measured fluorometrically.

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All products and services are for Research Use Only. Do Not use in humans.

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