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GSH/GSSG Assay Kit (CAT#: CB-P050-K) Datasheet

Glutathione is a tripeptide of glycine, glutamate and cysteine, and is one of the key antioxidants that protect cells from damage by reactive oxygen species. Glutathione (GSH) reduces the disulfide bonds in cytoplasmic proteins to cysteine, where it is converted to oxidized GSSG.

Creative Biolabs' GSH/GSSG Assay Kit is designed to accurately measure total, reduced and oxidized glutathione in biological samples using an enzymatic method using Ellman reagent (DTNB) and glutathione reductase (GR). DTNB reacts with reduced glutathione to form a yellow product. The rate of change of optical density measured at 412 nm is directly proportional to the concentration of glutathione in the sample. The kit can also measure oxidation (GSSG) by using a specific protocol that first removes all GSH with 1-methyl-2-vinylpyridinium triflate.
Sensitive and accurate. The linear detection range is 0.01-3 uM GSH equivalent, and the detection limit is 10 nM GSH equivalent.
For quantitative determination of reduced and oxidized glutathione (GSH / GSSG).
100 tests
Detection Method
Detection Limit
10 nM
Whole blood, plasma, serum, urine, tissue and cell extracts
Species Reactivity
All species
Protocol Length
20 min
Gel Pack
Shelf Life
6 months
Research Use
For Research Use Only! Not For Use in Humans.

All products and services are for Research Use Only. Do Not use in humans.


Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

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