Description
Glutathione is a tripeptide of glycine, glutamate and cysteine, and is one of the key antioxidants that protect cells from damage by reactive oxygen species. Glutathione (GSH) reduces the disulfide bonds in cytoplasmic proteins to cysteine, where it is converted to oxidized GSSG.
Creative Biolabs' GSH/GSSG Assay Kit is designed to accurately measure total, reduced and oxidized glutathione in biological samples using an enzymatic method using Ellman reagent (DTNB) and glutathione reductase (GR). DTNB reacts with reduced glutathione to form a yellow product. The rate of change of optical density measured at 412 nm is directly proportional to the concentration of glutathione in the sample. The kit can also measure oxidation (GSSG) by using a specific protocol that first removes all GSH with 1-methyl-2-vinylpyridinium triflate.
Features
Sensitive and accurate. The linear detection range is 0.01-3 uM GSH equivalent, and the detection limit is 10 nM GSH equivalent.
Applications
For quantitative determination of reduced and oxidized glutathione (GSH / GSSG).
Size
100 tests
Detection Method
OD412nm
Detection Limit
10 nM
Sample
Whole blood, plasma, serum, urine, tissue and cell extracts
Protocol Length
20 min
Shipping
Gel Pack
Storage
-20°C
Shelf Life
6 months
Research Use
For Research Use Only! Not For Use in Humans.
