Glycan Standard 13C Biantennary bA2F 2x(Sial α-2,6) [Glycan Standards] (CAT#: CB-X013-K) Datasheet

Product Type
Glycan Standards
Description
N-Glycan labeled with 2 13 C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).
Features
Alternative glycan names: FA2BG2S2, F(6)A2BG2S2
Exact Mass: 2581.9538
13C- isotopic purity: 99.1%
Purity: 82% (UPLC-FLD after 2-AB labeling)
Advantages:
- Label-free glycan quantification by MS
- Absolute quantification of individual glycans in complex mixtures (e.g. glycan biomarkers)
- No external calibration required, use as internal standard due to stable isotope enrichment
- Standards are quantified by qNMR
- Custom-made software for rapid automated quantification available
- Stable isotope enrichment in glycan core and antennae for fragment quantification
- Degree of isotopic enrichment can be tuned during synthesis
- Standards provided as single isomers, no isobaric mixtures
- Fully synthetic and characterized by NMR, HPLC and MS, high purity (>95%)
- Custom synthesis of standards offered
- Compatible with labeling by reductive amination, permethylation, sialic acid derivatisation
Applications
- Measure serum glycan levels as disease markers by MS
- Quantify absolute and relative glycosylation in biopharmaceutical development and quality control
- Glycan biomarker discovery by MALDI (cross-quantification)
- Increase reproducibility in Lab-to-lab method transfer (internal calibration standard)
- De-convolute and quantify co-eluting peaks in LC-MS
- Quantify glycan recovery after sample preparation
- Protein characterization
Size
3×200 pmol
Sample Volume
30 µl
Handling Advice
Upon arrival store standards solutions at 4 °C.
Centrifuge the vial before opening at 1,000 x g for 1 minute. (To dislodge any material that may be dispersed on the wall or cap of
the vial).
Add 10 µL of the labeled glycan standard (200 pmol) to the glycan mixture and re-cap the vial immediately.
For MALDI applications is recommended to add any Na+ ion rich reagent or K+ suppression reagent as NaOH or sodium citrate to
avoid glycan K+ adducts in the MALDI spectra.
Storage
Stored at 4 °C standards are stable>1 year without significant loss of purity
Shelf Life
>1 yr at 4 °C.
Research Use
For research use only. Not for human or drug use

Figure 1 Structure

All products and services are for Research Use Only. Do Not use in humans.

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