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Reduced Glutathione (GSH) Assay Kit (Fluorometric) (CAT#: CB-P051-K) Datasheet

Description
Glutathione (GSH) is a thiol-containing tripeptide, which is a key antioxidant in living systems. The state of GSH in the cell seems to be a sensitive indicator of the overall health of the cell. Creative Biolabs' reduced glutathione analysis kit is based on a specific enzymatic cycling method in the presence of GSH and fluorophores. The reduction of the fluorophore produces a stable fluorescent product whose fluorescence is directly proportional to the amount of GSH in the sample and can be tracked kineticly (Ex / Em = 535/587 nm). Our detection method is the most specific and sensitive detection method on the market. Oxidized glutathione will not interfere with the measurement. The method is simple and reproducible, and can specifically detect reduced glutathione (GSH) as low as 8 pmol/well in a 100 µl reaction.
Features
•Simple procedure; takes only ~1-2 hours
•Fast and convenient
•Specific: does not detect oxidized GSSG
•Sensitive: detects as low as 8 pmol GSH
Applications
The Colorimetric Glutathione Detection Kit provides a simple in vitro assay for detecting reduced GSH levels in apoptosis and other pathological processes. Oxidized GSSG is not detected.
Size
100 assays
Detection Method
Fluorescence (535/587 nm)
Sample
Whole blood, plasma, serum, urine, tissue and cell extracts
Species Reactivity
Mammalian
Components
GSH Assay Buffer (in DMSO)
Substrate Mix A
Substrate Mix B
Enzyme Mix A
Enzyme Mix B
Enzyme Mix C
Sulfosalicylic Acid (SSA, 1 gram)
GSH Standard
Shipping
Gel Pack
Storage
-20°C
Shelf Life
6 months
Research Use
For Research Use Only! Not For Use in Humans.

Figure: (a) GSH Standard Curve, results from multiple experiments. (b) Assay Specificity: measurement of GSH (100 pmol) and GSSG (1000 pmol). The kit can effectively discriminate reduced GSH and oxidized GSSG forms.

All products and services are for Research Use Only. Do Not use in humans.

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