Reduced Glutathione (GSH) Assay Kit (Fluorometric) (CAT#: CB-P051-K) Datasheet

Glutathione (GSH) is a thiol-containing tripeptide, which is a key antioxidant in living systems. The state of GSH in the cell seems to be a sensitive indicator of the overall health of the cell. Creative Biolabs' reduced glutathione analysis kit is based on a specific enzymatic cycling method in the presence of GSH and fluorophores. The reduction of the fluorophore produces a stable fluorescent product whose fluorescence is directly proportional to the amount of GSH in the sample and can be tracked kineticly (Ex / Em = 535/587 nm). Our detection method is the most specific and sensitive detection method on the market. Oxidized glutathione will not interfere with the measurement. The method is simple and reproducible, and can specifically detect reduced glutathione (GSH) as low as 8 pmol/well in a 100 µl reaction.

•Simple procedure; takes only ~1-2 hours
•Fast and convenient
•Specific: does not detect oxidized GSSG
•Sensitive: detects as low as 8 pmol GSH

The Colorimetric Glutathione Detection Kit provides a simple in vitro assay for detecting reduced GSH levels in apoptosis and other pathological processes. Oxidized GSSG is not detected.