Glycan Standard ¹³C Biantennary A1(6)F (Sial α-2,3) [Glycan Standards] (CAT#: CB-X019-K) Datasheet

Glycan Standards

N-Glycan labeled with 2 ¹³ C atoms per GlcNAc, for use as a quantitative standard in applications such as Matrix-assisted Laser Desorption Ionization-time-of-flight (MALDI-TOF), Electrospray Ionization (ESI) and Liquid Chromatography Mass Spectrometry (LC-MS).

Alternative glycan names: A1F, G2FS1, FA2G2S1, F(6)A2G2S1, G2A1(6)F
Exact Mass: 1923.6607
Molecular Formula: C6513C8H128N5O53
13C- isotopic purity: 99.1%
Purity: 95% (UPLC-FLD after 2-AB labeling)
Advantages:
- Label-free glycan quantification by MS
- Absolute quantification of individual glycans in complex mixtures (e.g. glycan biomarkers)
- No external calibration required, use as internal standard due to stable isotope enrichment
- Standards are quantified by qNMR
- Custom-made software for rapid automated quantification available
- Stable isotope enrichment in glycan core and antennae for fragment quantification
- Degree of isotopic enrichment can be tuned during synthesis
- Standards provided as single isomers, no isobaric mixtures
- Fully synthetic and characterized by NMR, HPLC and MS, high purity (>95%)
- Custom synthesis of standards offered
- Compatible with labeling by reductive amination, permethylation, sialic acid derivatisation

- Measure serum glycan levels as disease markers by MS
- Quantify absolute and relative glycosylation in biopharmaceutical development and quality control
- Glycan biomarker discovery by MALDI (cross-quantification)
- Increase reproducibility in Lab-to-lab method transfer (internal calibration standard)
- De-convolute and quantify co-eluting peaks in LC-MS
- Quantify glycan recovery after sample preparation
- Protein characterization