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Glucose Oxidase Assay Kit (Fluorometric) (CAT#: CB-P184-K) Datasheet

Product Type
Kit
Description
The glucose oxidase assay kit uses a fast and sensitive Fluorometric assay to measure glucose oxidase activity. It can be carried out in 96-well or 384-well microtiter plate format, and can be easily automated without separation steps.
The glucose oxidase assay protocol uses a red dye, which can realize a dual recordable mode. The Fluorometric signal can be easily read by the Fluorometric microplate reader with Ex / Em = 540/590 nm or the absorbance microplate reader with 576 nm.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Sensitivity
0.05 mU/ml
Sample
Urine, Plasma, Other biological fluids
Species Reactivity
Mammals
Protocol Length
2h 00m
Components
AbRed Indicator 1 vial
Assay Buffer 1 x 50ml
DMSO 1 x 200µl
Glucose oxidase 1 vial
Glucose standard 1 vial
Horseradish Peroxidase 1 vial
Requires
Microplate reader
Handling Advice
Glucose oxidase assay protocol summary:
- add standards and samples to wells
- add reaction mix to wells and incubate for 10-30 min at 37°C
- analyze with a microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Glucose oxidase dose response was measured in a solid black 96-well plate using a microplate reader. As low as 0.05 mU/ ml glucose oxidase was detected with 30 minutes incubation (n=3).

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All products and services are for Research Use Only. Do Not use in humans.

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