Free Glycerol Assay Kit (Fluorometric), High Sensitivity (CAT#: CB-P159-K) Datasheet

Product Type
Kit
Description
The free glycerol test kit is a simple, sensitive and easy-to-use tool that can detect small amounts of glycerol.
This assay is suitable for measuring trace amounts of glycerol in samples containing reducing substances, which may interfere with oxidase-based assays. In the assay, glycerol reacts with the enzyme mixture to form an intermediate, which is then oxidized and produces Fluorometric. The Fluorometric intensity is proportional to the content of glycerin.
The test kit can detect less than 40 pmol of glycerol.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric
Sensitivity
0.04 nmol/well
Sample
Cell Lysate, Tissue Lysate
Species Reactivity
Mammals
Protocol Length
0h 30m
Components
Glycerol Assay Buffer 1 x 25ml
Glycerol Developer 1 vial
Glycerol Enzyme Mix (lyophilized) 1 vial
Glycerol Standard 1 x 0.2ml
PicoProbe 1 x 0.4ml
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Example of Glycerol standard curve following assay's protocol.


Figure 2 Functional Studies- Example of Glycerol standard curve following assay's protocol.


Figure 3 Functional Studies
Measurement of Glycerol Levels

All products and services are for Research Use Only. Do Not use in humans.

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