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Galactose Assay Kit (Colorimetric/Fluorometric) (CAT#: CB-P176-K) Datasheet

Product Type
Kit
Description
The galactose assay kit (colorimetry/Fluorometric) provides a convenient for directly measuring the galactose levels in various biological samples (such as serum, plasma and other body fluids, food and growth media). In this assay, the oxidation of galactose produces a product that reacts with the galactose probe to produce color (OD570nm) or Fluorometric (Ex / Em 535/587 nm). Liquid samples can be tested directly without purification. The assay is fast, convenient and sensitive. It can also be used for high-throughput analysis.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Sensitivity
< 10 nmol/well
Sample
Tissue, Adherent cells, Suspension cells, Tissue Homogenate
Species Reactivity
Mammals
Protocol Length
0h 40m
Components
Galactose Assay Buffer 1 x 25ml
Galactose Enzyme Mix (Lyophilized) 1 vial
Galactose Probe (DMSO solution) 1 x 200µl
Galactose Standard (100 nmol/ul) 1 x 100µl
HRP (Lyophilized) 1 vial
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Galactose measured in cell lysates showing quantity (nmol) per 1 mln cells.

Figure 2 Fluorometric standard curve: mean of duplicates (+/- SD) with background reads subtracted.

Figure 3 Galactose levels measured in biological fluids showing quantity (nmol) per mL. Samples tested undiluted.

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All products and services are for Research Use Only. Do Not use in humans.

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