Connect with Us at the Upcoming Events

With in-person events back again, Creative Biolabs is excited to greet you at the upcoming international conferences. Meet our team at 2022 BIO International Convention in June (booth #1754) and at the 13th Annual World ADC San Diego in September (booth number to be updated) for expert consultation on your drug discovery. Shoot an email to arrange an in-person meeting!

Book a Meeting

Ficolin-3 ELISA kit [Human]-1 x 96 det. (CAT#: CB-P087-K) Datasheet

Product Type
Kit
Description
Fibrin is a group of proteins that contain both a collagen-like domain and a fibrinogen-like domain. Three forms of Feikelin have been identified in humans: Feikelin 1 (M-ficolin), Feikelin 2 (L-Ficolin) and Feikelin 3 (H-Ficolin 3). Ficolin-3 (also known as Bodo antigen or H-ficolin) consists of a collagen-like chain and three C-terminal recognition domains that bind to acetyl groups on the surface of microorganisms (such as GlcNAc or GalNAc). Ficolin-3 is synthesized in the liver (from where it is secreted into the blood circulation) and the lungs. Similar to MBL and Ficolin-2, Ficolin-3 relies on MBL-related serine protease 2 (MASP-2) to activate the complement system. After the Ficolin-3 / MASP-2 complex binds to the surface of the microorganism, MASP-2 cleaves C4 and C2 in turn, thereby producing the C3 convertase C4bC2b, which ultimately leads to opsonization, direct lysis of pathogens and recruitment of inflammatory cells. The average concentration of Ficolin-3 in serum is 15μg/ml, with only a small change. Except for some patients with systemic lupus erythematosus, Ficolin-3 is present in all serums of more than 150,000 people. It was found that about 5% of patients with systemic lupus erythematosus were Ficolin-3 negative, possibly due to the presence of anti-Ficolin-3 autoantibodies. Ficolin-3 is always present in 398 patients with other autoimmune diseases. In liver disease, serum levels decrease with increasing severity of cirrhosis. Ficolin-3 binds to late apoptotic cell populations, and at the same time a firm and uniform binding to necrotic cells is observed. The binding properties are different from MBL and Ficolin-2. The binding of Ficolin-3 to late apoptotic cells resulted in a significant increase in macrophage adhesion/uptake.
Applications
Human Ficolin-3 ELISA kit is used for the in vitro quantitative determination of human Ficolin-3 in serum, plasma and cell culture supernatant samples.
Principle
Human Ficolin-3 ELISA kit is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle. The working time is 3 hours. The effective format of two plates with twelve disposable 8-well strips allows free choice of batch size for determination. Samples and standards are captured by specific antibodies bound to the solid phase. The biotinylated tracer antibody will bind to the captured Ficolin-3. The streptavidin-peroxidase conjugate will bind the biotinylated tracer antibody. The streptavidin-peroxidase conjugate will react with the substrate tetramethylbenzidine (TMB). The enzyme reaction is stopped by adding oxalic acid. Measure the absorbance at 450nm with a spectrophotometer. Draw a standard curve by plotting the absorbance (linear) against the corresponding concentration (logarithm) of the Ficolin-3 standard. The concentration of the human Ficolin-3 sample that runs simultaneously with the standard can be determined from the standard curve.
Size
1 x 96 det.
Detection Method
Enzyme immunoassay (ELISA) technique
Detection Limit
3-500 ng/ml
Sensitivity
3 ng/ml
Sample
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Specimen Collection
Blood samples were collected using sterile venipuncture techniques, and serum was obtained using standard procedures. At least 5 ml of whole blood is recommended. Let the blood clot in the serum test tube for 60-65 minutes at room temperature (20-25°C). Centrifuge the blood sample and transfer the cell-free serum to a clean test tube. The serum must be handled correctly to prevent complement activation in vitro. Serum should be frozen in a sealed tube at -70°C or lower for long-term storage or transport on dry ice. The sample should not be frozen and thawed more than once. Do not use jaundice, lipemia and hemolytic serum. You cannot use heat-inactivated serum. Plasma cannot be used.
Sample Volume
100 µl/well
Species Reactivity
Human
Cross Reactivity
Bovine - No, Canine - No, Goat - No, Horse - No, Mouse - No, Pig - No, Rabbit - No, Rat - No, Sheep - No
Protocol Length
2 hours
Storage
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.
Research Use
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.

All products and services are for Research Use Only. Do Not use in humans.

ONLINE INQUIRY

Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

  • *
  • *
  • *
USA

UK