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Endo-β-Galactosidase [For Glycan Release] (CAT#: CB-P261-K) Datasheet

Product Type
Kit
Description
Endo-β-galactosidase cleaves internal β(1-4) galactose bonds in the unbranched repeating poly-N-acetyllactosamine structure. Sulfated structures (such as keratan sulfate) are also cleaved. Substance branching and/or fucosylation can reduce or eliminate cleavage.
Endo-β-galactosidase can be used to identify and remove poly-N-acetyllactosamine structures on many biologically important glycoconjugates.
Features
Source: Recombinant from Bacteroides fragilis in E. Coli

EC: 3.2.1.103

Specific Activity:>140 U/mg

Activity:>14 U/mL

Molecular weight: ~32,000 daltons

Optimum pH: 5.8

Specific Activity:
One unit of Endo-β-Galactosidase is defined as the amount that will liberate one µmole of reducing sugar per minute at 37°C and pH 5.8 from bovine corneal keratan sulfate.

Purity: Endo-β-Galactosidase is tested for contaminating protease as follows: 10 µg of denatured BSA is incubated for 24 hours at 37°C with 2 µL of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation.
Applications
Endo-β-galactosidase cleaves internal β(1-4) galactose linkages in unbranched, repeating poly-N-acetyllactosamine structures.
Size
0.9 U / 60 µL; Sufficient for up to 60 reactions.
Sample
Glycoproteins and glycopeptides containing N-linked glycans
Components
60 µL aliquot of enzyme (0.9 U) in 20 mM tris-HCl, pH 7.5
1 vial reaction buffer- 250mM Sodium phosphate, pH 5.8
Handling Advice
For glycoproteins:
1. Add up to 100 µg of glycoprotein to a tube.
2. Add 4 µL 5X buffer and water to 19 µL.
3. Add 1 µL enzyme.
4. Incubate at 37°C for 2 hrs.
Storage
Store enzyme at 4°C.
Shelf Life
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Research Use
For research use only. Not for human or drug use

All products and services are for Research Use Only. Do Not use in humans.

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