Endo-β-Galactosidase [For Glycan Release] (CAT#: CB-P261-K) Datasheet

Kit

Endo-β-galactosidase cleaves internal β(1-4) galactose bonds in the unbranched repeating poly-N-acetyllactosamine structure. Sulfated structures (such as keratan sulfate) are also cleaved. Substance branching and/or fucosylation can reduce or eliminate cleavage.
Endo-β-galactosidase can be used to identify and remove poly-N-acetyllactosamine structures on many biologically important glycoconjugates.

Source: Recombinant from Bacteroides fragilis in E. Coli

EC: 3.2.1.103

Specific Activity:>140 U/mg

Activity:>14 U/mL

Molecular weight: ~32,000 daltons

Optimum pH: 5.8

Specific Activity:
One unit of Endo-β-Galactosidase is defined as the amount that will liberate one µmole of reducing sugar per minute at 37°C and pH 5.8 from bovine corneal keratan sulfate.

Purity: Endo-β-Galactosidase is tested for contaminating protease as follows: 10 µg of denatured BSA is incubated for 24 hours at 37°C with 2 µL of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation.

Endo-β-galactosidase cleaves internal β(1-4) galactose linkages in unbranched, repeating poly-N-acetyllactosamine structures.