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Endo-β-Galactosidase [For Glycan Release] (CAT#: CB-P261-K) Datasheet

Product Type
Kit
Description
Endo-β-galactosidase cleaves internal β(1-4) galactose bonds in the unbranched repeating poly-N-acetyllactosamine structure. Sulfated structures (such as keratan sulfate) are also cleaved. Substance branching and/or fucosylation can reduce or eliminate cleavage.
Endo-β-galactosidase can be used to identify and remove poly-N-acetyllactosamine structures on many biologically important glycoconjugates.
Features
Source: Recombinant from Bacteroides fragilis in E. Coli

EC: 3.2.1.103

Specific Activity:>140 U/mg

Activity:>14 U/mL

Molecular weight: ~32,000 daltons

Optimum pH: 5.8

Specific Activity:
One unit of Endo-β-Galactosidase is defined as the amount that will liberate one µmole of reducing sugar per minute at 37°C and pH 5.8 from bovine corneal keratan sulfate.

Purity: Endo-β-Galactosidase is tested for contaminating protease as follows: 10 µg of denatured BSA is incubated for 24 hours at 37°C with 2 µL of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation.
Applications
Endo-β-galactosidase cleaves internal β(1-4) galactose linkages in unbranched, repeating poly-N-acetyllactosamine structures.
Size
0.9 U / 60 µL; Sufficient for up to 60 reactions.
Sample
Glycoproteins and glycopeptides containing N-linked glycans
Components
60 µL aliquot of enzyme (0.9 U) in 20 mM tris-HCl, pH 7.5
1 vial reaction buffer- 250mM Sodium phosphate, pH 5.8
Handling Advice
For glycoproteins:
1. Add up to 100 µg of glycoprotein to a tube.
2. Add 4 µL 5X buffer and water to 19 µL.
3. Add 1 µL enzyme.
4. Incubate at 37°C for 2 hrs.
Storage
Store enzyme at 4°C.
Shelf Life
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Research Use
For research use only. Not for human or drug use

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All products and services are for Research Use Only. Do Not use in humans.

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