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DPP4 Inhibitor Screening Assay Kit (Fluorescent) (CAT#: CB-P166-K) Datasheet

Product Type
Kit
Description
The dipeptidyl peptidase IV (DPP4) inhibitor screening assay kit provides a convenient Fluorometric-based for screening DPP4 inhibitors. The assay uses the fluorescent substrate Gly-Pro-aminomethylcoumarin (AMC) to measure DPP4 activity. The cleavage of the peptide bond by DPP releases free AMC groups, resulting in Fluorometric that can be analyzed with an excitation wavelength of 350-360 nm and an emission wavelength of 450-465 nm.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Sample
Cell Lysate
Species Reactivity
Mammals
Protocol Length
0h 20m
Components
96-Well Plate Cover 1 x 1ml
DPP Assay Buffer (10X) 1 x 10ml
DPP IV (human recombinant) 1 vial
DPP Substrate 1 vial
Half Volume 96-Well Solid Plate (white) 1 x 17ml
Sitagliptin Positive Control Inhibitor 1 x 1ml
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Inhibition of DPP (IV) by Sitagliptin. Vehicle represents 100% initial activity.

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All products and services are for Research Use Only. Do Not use in humans.

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