CreaClean™ EB10 Glycan Clean-up Cartridges [For Glycan Clean Up] (CAT#: CB-P277-K) Datasheet

Product Type
Kit
Description
The cartridge contains a unique non-porous electronic interaction (EI) matrix. This is like a superhydrophobic resin that can even bind very hydrophilic polysaccharides. Most of the salt and detergent are either simply passed through the cartridge or gently bound together, which can be washed off before eluting the glycans.
Features
Binding Capacity: Each CreaClean™ EB10 cartridge can typically bind up to 50 g of O- or N-linked
glycans.
Number of Samples: CreaClean™ EB10 cartridges are designed for single use only.
Suitable Samples: A wide range of glycans can be purified. These include N-linked and O-linked type
oligosaccharides, tri-saccharides and larger structures.
The cartridges are not suitable either for monosaccharides or disaccharides which are
generally bound too weakly for efficient purification or for large linear poly-sialylated
glycans which can be bound very tightly to the resin.
Glycan samples must be applied to the cartridges in solutions that are substantially
aqueous.
Structural Integrity: No detectable (< 2 mole per cent) loss of sialic acid, fucose, sulfate, or phosphate.
Binding efficiency:> 95 % for most glycans
Applications
For purification of glycans from a variety of complex mixtures (including removal of salts and detergents).
Sample
Glycoproteins and glycopeptides containing N-linked glycans
Requires
• Pure water (HPLC grade)
• Methanol
• 1M sodium hydroxide (aq)
• 30% acetic acid (aq)
• Acetonitrile (HPLC grade)
• Trifluoroacetic acid (Analar grade)
• Wash A: 5 % (v/v) acetonitrile (aq) plus 0.1% trifluoroacetic acid
• Wash B: 50 % (v/v) acetonitrile (aq) plus 0.1% trifluoroacetic acid
• Pipettes
• Microcentrifuge
Handling Advice
Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrate.
Shipping
The product should be shipped at ambient temperature.
Storage
Store at room temperature in the dark. Protect from sources of heat, light, and moisture. The cartridges are stable for at least two years as supplied.
Shelf Life
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Research Use
For research use only. Not for human or drug use

All products and services are for Research Use Only. Do Not use in humans.

ONLINE INQUIRY

Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

  • *
  • *
  • *
USA

UK