Free Glycerol Assay Kit (Colorimetric/Fluorometric) (CAT#: CB-P153-K) Datasheet

Product Type
Kit
Description
The glycerol assay kit provides a sensitive and simple to measure the concentration of free glycerol. In the glycerol assay protocol, glycerol is oxidized by the enzyme to produce a product, which reacts with the probe to produce color (λ=570 nm) and Fluorometric (Ex / Em = 535/587 nm). This glycerol assay kit can detect 50 pmol-10 nmol (or ~1-10000 µM range) of glycerol. "
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Detection Limit
1 µM - 10000 µM
Sensitivity
> 1 µM
Sample
Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
Species Reactivity
Mammals
Protocol Length
0h 40m
Components
Glycerol Assay Kit provides a sensitive, easy assay to measure free glycerol concentration.
In the glycerol assay protocol, glycerol is enzymatically oxidized to generate a product which reacts with the probe to generate color (λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm).
This glycerol assay kit can detect 50 pmol-10 nmol (or ~1-10000 µM range) of glycerol.
Requires
Microplate reader
Handling Advice
Glycerol assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min
- analyze with a microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Standard curve: mean of duplicates (+/- SD) with background reads subtracted


Figure 2 Standard curve: mean of duplicates (+/- SD) with background reads subtracted


Figure 3 Functional Studies
Free Glycerol measured in cell culture lysates showing quantity (nmol) per 1 mln cells.


Figure 4 Functional Studies
a. Measurement of free glycerol in pooled human serum (10 μL). b. Measurement of free glycerol in rat liver lysate (500 μg).

All products and services are for Research Use Only. Do Not use in humans.

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