Product Type
Kit
Description
The glycerol assay kit provides a sensitive and simple to measure the concentration of free glycerol. In the glycerol assay protocol, glycerol is oxidized by the enzyme to produce a product, which reacts with the probe to produce color (λ=570 nm) and Fluorometric (Ex / Em = 535/587 nm). This glycerol assay kit can detect 50 pmol-10 nmol (or ~1-10000 µM range) of glycerol. "
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Detection Limit
1 µM - 10000 µM
Sensitivity
> 1 µM
Sample
Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
Protocol Length
0h 40m
Components
Glycerol Assay Kit provides a sensitive, easy assay to measure free glycerol concentration.
In the glycerol assay protocol, glycerol is enzymatically oxidized to generate a product which reacts with the probe to generate color (λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm).
This glycerol assay kit can detect 50 pmol-10 nmol (or ~1-10000 µM range) of glycerol.
Requires
Microplate reader
Handling Advice
Glycerol assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min
- analyze with a microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use
