CreaClean™ Post-glycosidase Clean-up Plate [For Glycan Clean Up] (CAT#: CB-P282-K) Datasheet

Kit

The plate is a 96-well membrane bottom plate, which contains a dedicated protein binding membrane with a nominal pore size of 0.45 µm.

Number of Samples: Sufficient for up to 96 samples.
Amount of Sample: Up to 350 µL per well
Suitable Samples: Underivatised glycans released enzymatically from glycoproteins including acidified N-glycans released from glycoproteins

Post-exoglycosidase clean-up

Used to remove exoglycosidase and other protein and peptide contaminants from unlabeled glycan mixtures. This can prevent the HPLC column from being contaminated during subsequent chromatographic analysis. The plate can also be used to remove proteins prior to mass spectrometry analysis of glycans.


Post-endoglycosidase Clean-up
It is used to remove endoglycosidase and other proteins and peptide substances after enzymatic release of glycans from glycoproteins. Using this protein binding membrane will provide a cleaner sample and increase the signal for subsequent fluorescent labeling of glycans. The plate can also be used to remove enzymes and other protein and peptide substances before permethylation of glycans and/or mass spectrometry.