CreaClean™ Post-glycosidase Clean-up Plate [For Glycan Clean Up] (CAT#: CB-P282-K) Datasheet

Product Type
Kit
Description
The plate is a 96-well membrane bottom plate, which contains a dedicated protein binding membrane with a nominal pore size of 0.45 µm.
Features
Number of Samples: Sufficient for up to 96 samples.
Amount of Sample: Up to 350 µL per well
Suitable Samples: Underivatised glycans released enzymatically from glycoproteins including acidified N-glycans released from glycoproteins
Applications
Post-exoglycosidase clean-up

Used to remove exoglycosidase and other protein and peptide contaminants from unlabeled glycan mixtures. This can prevent the HPLC column from being contaminated during subsequent chromatographic analysis. The plate can also be used to remove proteins prior to mass spectrometry analysis of glycans.


Post-endoglycosidase Clean-up
It is used to remove endoglycosidase and other proteins and peptide substances after enzymatic release of glycans from glycoproteins. Using this protein binding membrane will provide a cleaner sample and increase the signal for subsequent fluorescent labeling of glycans. The plate can also be used to remove enzymes and other protein and peptide substances before permethylation of glycans and/or mass spectrometry.
Sample
Glycoproteins and glycopeptides containing N-linked glycans
Components
CreaClean 96 well Protein Binding Membrane Plate 1
Plate Lid 1
Handling Advice
Ensure that any glass, plastic ware or solvents used with this item are free of environmental carbohydrates. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrate. once used, the plate should be discarded according to local safety rules
Storage
Store at room temperature. Protect from sources of heat, light, and moisture. When stored correctly, the reagents should be stable for 36 months from date of purchase.
Shelf Life
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Research Use
For research use only. Not for human or drug use

All products and services are for Research Use Only. Do Not use in humans.

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