CreaClean™ CEX Glycan Clean-up Cartridges [For Glycan Clean Up] (CAT#: CB-P279-K) Datasheet

Product Type
Kit
Description
The cartridge contains a cation exchange resin stored in 1 M hydrochloric acid.
Features
Binding Capacity: Each CreaClean™ CEX cartridge can typically clean up O-glycans released from
1000 µg of glycoprotein.
Number of Samples: CreaClean™ CEX cartridges are designed for single use only.
Suitable Samples: A wide range of O-glycans can be purified including di-saccharides, tri-saccharides and
oligosaccharides. Glycan samples must be applied to the cartridges in solutions that
are substantially aqueous.
Structural Integrity: No detectable loss of sialylated glycans.
Binding Selectivity: Essentially stoichiometric binding and elution for most complex glycan mixtures.
Applications
For purification of O-glycans from non-carbohydrate material including salts, proteins, and detergents. Applications include cleanup of glycans following hydrazinolysis or any other non-reductive O-glycan release method.
Sample
Glycoproteins and glycopeptides containing N-linked glycans
Requires
• Pure water: resistivity 18 MΩ-cm, particle free (>0.22 μm), TOC <10 ppb
• Pipettes
• pH paper
• Centrifugal evaporator
Handling Advice
Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrate.
Shipping
The product should be shipped at ambient temperature.
Storage
Store refrigerated at 4 to 10°C in the dark. Protect from sources of heat, light, and moisture. The cartridges are stable for at least 6 months from date of manufacture.
Shelf Life
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Research Use
For research use only. Not for human or drug use

All products and services are for Research Use Only. Do Not use in humans.

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