Complement factor I ELISA Kit [Human], 1 x 96 det. (CAT#: CB-P135-K) Datasheet

Kit

CFI is a protein of the complement system (serine protease), also known as C3b/C4f inactivator, and is a protein encoded by the CFI gene located on chromosome 4. It regulates complement activation by cutting cell binding or liquid phases C3b and C3b. C4b. CFI is mainly synthesized in the liver and is initially secreted as a single 88 kDa gene product. Then, the precursor protein is cleaved by furin to produce a mature CFI protein, which is the disulfide of heavy chain residues (residues 19-335, 51 kDa) and light chain (residues 340-583, 37 kDa) Bonded dimers. Only mature proteins are active. Genetic polymorphisms in CFI have been observed (variants R201S, R406H, R502L). Because the activation of the alternative pathway of complement is not controlled, CFI deficiency can lead to a decrease in the level of complement component 3 (C3) in the plasma, and is associated with repeated bacterial infections in children. Recently, it has been proved that mutations in the CFI gene are related to the development of hemolytic uremic syndrome, which is also a kidney disease caused by unregulated complement activation.

Complement factor I ELISA kit is used for the in vitro quantitative determination of human CFI in plasma and serum.

Complement factor I ELISA kit is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle. The working time is 3 hours. The effective form of the plate with twelve disposable 8-well plates allows free choice of batch size for determination. The samples and standards are incubated in microtiter wells coated with antibodies that recognize human CFI. The biotinylated tracer antibody will bind to the captured human CFI. The streptavidin-peroxidase conjugate will bind the biotinylated tracer antibody. The streptavidin-peroxidase conjugate will react with the substrate tetramethylbenzidine (TMB). The enzyme reaction is stopped by adding oxalic acid. Measure the absorbance at 450nm with a spectrophotometer. Draw a standard curve by plotting the absorbance (linear) relative to the corresponding concentration (logarithm) of the human CFI standard. The human CFI concentration of samples run at the same time as the standard can be determined from the standard curve.