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Creative Biolabs is excited to greet you at the upcoming international conferences. Meet our team at the 13th Annual World ADC San Diego and the 13th Annual World Multispecific Summit in September, the 10th Annual Immuno-Oncology Summit in October, and the Antibody Engineering & Therapeutics (US) 2022 in December for expert consultation on your drug discovery projects. Shoot an email to arrange an in-person meeting!

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Cellular Reactive Oxygen Species (ROS) Assay Kit (CAT#: CB-P048-K) Datasheet

The ROS detection and analysis kit is a highly sensitive and safe detection method for detecting reactive oxygen species. ROS includes many reactive molecules and free radicals derived from molecular oxygen, which damage DNA and RNA and oxidize proteins and lipids (lipid peroxidation). The most common ROS include superoxide anion (O2-), hydrogen peroxide (H2O2), hydroxyl radical (HO-) and singlet oxygen (1O2), all of which are more reactive than oxygen (O2) itself. Molecules are produced during the electron transport process of aerobic respiration in the mitochondria, or through oxidation-reduction enzymes and metal-catalyzed oxidation.

The ROS detection and analysis kit uses the cell permeation reagent Dichlorodihydrofluorescein-diacetate (H2DCFDA), which is a fluorescent dye that can measure the intracellular hydroxyl, hydrogen peroxide and other reactive oxygen species (ROS) activity. After diffusion into the cell, the acetyl group on H2DCFDA is cleaved by intracellular esterase to generate a non-fluorescent compound, which is rapidly oxidized by ROS to highly fluorescent 2',7'-Dichlorodihydrofluorescein. The fluorescence intensity is directly proportional to the level of ROS in the cytosol.
•Fluorescence-based measurement: Ex / Em = 485/530 nm
•Based on the unique fluorescent probe H2DCFDA.
•Quick and easy agreement.
•Safety: No radioactivity, no special treatment required.
•High throughput.
•Accurate and repeatable results.
Measurement of intracellular levels of ROS
Microplate reader (Ex/Em = 485/530 nm)
Microscope with TRITC channel
Flow Cytometry: FL1 Channel
5×96 assays
Whole blood, plasma, serum, urine, tissue and cell extracts
Species Reactivity
5 mg Fluorescent Substrate (H2DCFDA)
50 mL 10X Assay Buffer
500 μL Positive control (H2O2, 8.8M)
Gel Pack
4 °C.
Shelf Life
12 months
Research Use
For Research Use Only! Not For Use in Humans.

All products and services are for Research Use Only. Do Not use in humans.


Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

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