ATPase/GTPase Assay Kit (Colorimetric) (CAT#: CB-P002-K) Datasheet

Creative Biolabs' ATPase / GTPase analysis kit provides a highly sensitive method to determine the activity of ATPase / GTPase in the form of a microplate. Its proprietary formula has a single reagent that can accurately measure enzyme activity within 30 minutes at room temperature. The modified malachite green reagent forms a stable dark green with free phosphate, which is measured on a microplate reader (600-660 nm).

High sensitivity: 0.007 U/L ATPase or GTPase activity is detected.
Fast and convenient: a single reagent, homogeneous "mixed measurement" assay can quantify enzyme activity within 30 minutes.
Rugged and durable, suitable for HTS: detection at 620nm can greatly reduce the potential interference of colored compounds. Z'factor> 0.7 was observed in 96-well plates and 384-well plates. It can be easily automated on the HTS liquid handling system.

The kit is used to quantitatively determine the activity of ATPase or GTPase and to screen its inhibitors with high throughput.