Description
Creative Biolabs' ATPase / GTPase analysis kit provides a highly sensitive method to determine the activity of ATPase / GTPase in the form of a microplate. Its proprietary formula has a single reagent that can accurately measure enzyme activity within 30 minutes at room temperature. The modified malachite green reagent forms a stable dark green with free phosphate, which is measured on a microplate reader (600-660 nm).
Features
High sensitivity: 0.007 U/L ATPase or GTPase activity is detected.
Fast and convenient: a single reagent, homogeneous "mixed measurement" assay can quantify enzyme activity within 30 minutes.
Rugged and durable, suitable for HTS: detection at 620nm can greatly reduce the potential interference of colored compounds. Z'factor> 0.7 was observed in 96-well plates and 384-well plates. It can be easily automated on the HTS liquid handling system.
Applications
The kit is used to quantitatively determine the activity of ATPase or GTPase and to screen its inhibitors with high throughput.
Size
200 tests
Detection Method
OD620nm
Detection Limit
0.007 U/L
Sample
ATPase and GTPase
Protocol Length
30 min
Components
Reagent: 50 mL Assay Buffer: 10 mL Standard: 1mL 1 mM phosphate
Shipping
Gel Pack
Storage
The reagents and standard are stable for one year when stored at 4°C
Shelf Life
12 months
Research Use
For Research Use Only! Not For Use in Humans.
