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ATPase/GTPase Assay Kit (Colorimetric) (CAT#: CB-P002-K) Datasheet

Creative Biolabs' ATPase / GTPase analysis kit provides a highly sensitive method to determine the activity of ATPase / GTPase in the form of a microplate. Its proprietary formula has a single reagent that can accurately measure enzyme activity within 30 minutes at room temperature. The modified malachite green reagent forms a stable dark green with free phosphate, which is measured on a microplate reader (600-660 nm).
High sensitivity: 0.007 U/L ATPase or GTPase activity is detected.
Fast and convenient: a single reagent, homogeneous "mixed measurement" assay can quantify enzyme activity within 30 minutes.
Rugged and durable, suitable for HTS: detection at 620nm can greatly reduce the potential interference of colored compounds. Z'factor> 0.7 was observed in 96-well plates and 384-well plates. It can be easily automated on the HTS liquid handling system.
The kit is used to quantitatively determine the activity of ATPase or GTPase and to screen its inhibitors with high throughput.
200 tests
Detection Method
Detection Limit
0.007 U/L
ATPase and GTPase
Species Reactivity
All species
Protocol Length
30 min
Reagent: 50 mL Assay Buffer: 10 mL Standard: 1mL 1 mM phosphate
Gel Pack
The reagents and standard are stable for one year when stored at 4°C
Shelf Life
12 months
Research Use
For Research Use Only! Not For Use in Humans.

All products and services are for Research Use Only. Do Not use in humans.


Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

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