Description
Creative Biolabs' ATP Analysis Kit (luminescence) provides a fast method to measure intracellular ATP. A single working reagent lyses the cells to release ATP. In the presence of luciferase, ATP immediately reacts with the substrate D-luciferin to produce light. Light intensity is a direct measure of the ATP concentration in the cell. This non-radioactive, homogeneous cell-based assay is performed in microplates. This reagent is compatible with all liquid handling systems and is suitable for high-throughput screening of 96-well and 384-well plates.
Features
•Safety. Non-radioactive determination.
•Sensitive and accurate. It can quantify as low as 0.02 uM ATP or a single cell.
•Similar and convenient. Type determination of "mixed cultivation measures". No washing and reagent transfer steps are involved.
•Robust and suitable for HTS: Z'factor> 0.5 is usually observed in 96-well plates and 384-well plates. It can be easily automated on the HTS liquid handling system.
Applications
Used for rapid, quantitative, bioluminescent determination of ATP and evaluation of the drug's effect on ATP metabolism.
Size
100 tests
Detection Method
Luminescence
Detection Limit
0.1 uM
Sample
Cells etc
Protocol Length
Get results in 10 minutes.
Components
Assay Buffer: 10 mL Substrate: 120 mL ATP Enzyme: 120 mL Standard: 100 mL 3 mM ATP
Shipping
Gel Pack
Storage
Store all reagents at -20°C.
Shelf Life
12 months after receipt.
Research Use
For Research Use Only! Not For Use in Humans.
