ATP Assay Kit (Luminescent) (CAT#: CB-P040-K) Datasheet

Creative Biolabs' ATP Analysis Kit (luminescence) provides a fast method to measure intracellular ATP. A single working reagent lyses the cells to release ATP. In the presence of luciferase, ATP immediately reacts with the substrate D-luciferin to produce light. Light intensity is a direct measure of the ATP concentration in the cell. This non-radioactive, homogeneous cell-based assay is performed in microplates. This reagent is compatible with all liquid handling systems and is suitable for high-throughput screening of 96-well and 384-well plates.

•Safety. Non-radioactive determination.
•Sensitive and accurate. It can quantify as low as 0.02 uM ATP or a single cell.
•Similar and convenient. Type determination of "mixed cultivation measures". No washing and reagent transfer steps are involved.
•Robust and suitable for HTS: Z'factor> 0.5 is usually observed in 96-well plates and 384-well plates. It can be easily automated on the HTS liquid handling system.

Used for rapid, quantitative, bioluminescent determination of ATP and evaluation of the drug's effect on ATP metabolism.