Product Type
Antibody
Description
The monoclonal antibody VIF9 recognizes rat surfactant protein D (SP-D). SP-D belongs to the collectin family. These proteins are oligomeric proteins composed of carbohydrate recognition domains (CRD) attached to the collagen region. Although they use a different CRD structure, they are similar in structure to ficolins: the C-type lectin domain of collectin. The antimicrobial effector mechanisms of SP-D are direct conditioning, neutralization and agglutination. Therefore, infection is limited and the subsequent adaptive immune response is coordinated at the same time. The lung is the main site of SP-D synthesis, where molecules are produced by type II alveolar cells and unbranched bronchial epithelial cells and secreted to the epithelial surface. SP-D was also found in different epithelial cells of the gastrointestinal tract and epithelial cells of exocrine glands. After inflammatory stress, the synthesis and secretion of SP-D increase significantly. In inflamed lungs, increased SP-D content in lavage fluid and tissues was found, especially in type II lung cells, Clara cells and hyperplastic goblet cells. The localization of SP-D in intracellular vesicles and lysosomal particles of alveolar macrophages indicates that receptor-mediated uptake has occurred. SP-D binds to apoptotic neutrophils and enhances its clearance rate through alveolar macrophages. The monoclonal antibody VIF9 specific to rat surfactant protein D has obvious cross-reactivity with human SP-D. Monoclonal antibody VIF9 stains paraffin sections of rat lungs. It cannot be used to stain paraffin sections of human lungs. It can also be used as a detection antibody for Elisa on human SP-D-coated wells.
Disease
Pulmonology
Size
100 µg
Immunogen
Purified Rat SP-D
Species
Rat
Cross reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
VIF9
Buffer
PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Application
Immuno assays, Paraffin sections, Western blot
Application Notes
The diluent used depends on the detection system used. It is recommended that users test reagents and determine their own optimal dilution. The typical initial working dilution is 1:50.
Storage Instructions
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Alternative Names
Pulmonary surfactant-associated protein D; PSP-D; CP-4; Lung surfactant protein D
