Anti-Rat SP-D Monoclonal Antibody (clone IVG8) -10 mg (CAT#: CB-P444-AB) Datasheet

Product Type
Antibody
Description
Monoclonal antibody IVG8 recognizes rat surfactant protein D (SP-D). SP-D belongs to the collectin family. These proteins are oligomeric proteins composed of carbohydrate recognition domains (CRD) attached to the collagen region. Although they use a different CRD structure, they are similar in structure to ficolins: the C-type lectin domain of collectin. The antimicrobial effector mechanisms of SP-D are direct conditioning, neutralization and agglutination. Therefore, infection is limited and the subsequent adaptive immune response is coordinated at the same time. The lung is the main site of SP-D synthesis, where molecules are produced by type II alveolar cells and unbranched bronchial epithelial cells and secreted to the epithelial surface. SP-D was also found in different epithelial cells of the gastrointestinal tract and epithelial cells of exocrine glands. After inflammatory stress, the synthesis and secretion of SP-D increase significantly. In inflamed lungs, increased SP-D content in lavage fluid and tissues was found, especially in type II lung cells, Clara cells and hyperplastic goblet cells. The localization of SP-D in intracellular vesicles and lysosomal particles of alveolar macrophages indicates that receptor-mediated uptake has occurred. SP-D binds to apoptotic neutrophils and enhances its clearance rate through alveolar macrophages. The monoclonal antibody IVG8 specific to rat surfactant D exhibits significant cross-reactivity with human SP-D. Monoclonal antibody IVG8 stains paraffin sections of rat lungs. It cannot be used to stain paraffin sections of human lungs. It can also be used as a detection antibody for Elisa on human SP-D-coated wells.
Disease
Pulmonology
Size
100 µg
Immunogen
Purified Rat SP-D
Species
Rat
Cross reactivity
Human
Host
Mouse
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
IVG8
Buffer
PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Application
Immuno assays, Paraffin sections, Western blot
Application Notes
For immunohistology and western blotting, diluents will be used, depending on the detection system used. It is recommended that users test reagents and determine their own optimal dilution. The typical initial working dilution is 1:10.
Storage Instructions
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Target
SP-D
Alternative Names
Pulmonary surfactant-associated protein D; PSP-D; CP-4; Lung surfactant protein D

All products and services are for Research Use Only. Do Not use in humans.

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