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Annexin V-EGFP Apoptosis Kit (CAT#: CB-P032-K) Datasheet

Description
The Annexin V-EGFP Apoptosis Detection Kit is based on the following observations: Most cell types transfer membrane phosphatidylserine (PS) from the inner surface of the plasma membrane to the cell surface soon after initiating apoptosis. Once on the cell surface, PS can be easily detected by staining with the enhanced green fluorescent protein (EGFP) fusion protein of Annexin V, which is a protein with a strong natural affinity for PS. The one-step dyeing process only takes 10 minutes. In addition, the assay can be performed directly on living cells. The detection results can be analyzed by flow cytometry or fluorescence microscope with FITC filter. EGFP is brighter than other fluorescent reagents and has higher light stability. When staining for annexin V-EGFP and PI at the same time, the kit can distinguish apoptosis and necrosis.
Features
•Simple one-step dyeing process, only 10 minutes
•Fast and convenient
•EGFP is a bright and light stable green fluorescent protein. When annexin V-EGFP and PI staining are performed at the same time, this kit can distinguish apoptosis and necrosis
Applications
Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Size
25 Assays
100 Assays
400 Assays
Detection Method
Flow cytometry (Ex = 488 nm; Em = 530 nm) and fluorescence microscopy
Sample
Works with both adherent and suspension cells.
Species Reactivity
Mammalian
Protocol Length
Get results in 9 minutes.
Components
Annexin V-EGFP
1X Binding Buffer
Propidium Iodide (PI)
Shipping
Gel Pack
Storage
4 °C.
Shelf Life
24 months from the date of purchase, if it is properly stored.
Research Use
For Research Use Only! Not For Use in Humans.

Figure: Detection of cell apoptosis with annexin V-EGFP. As shown in the figure, camptothecin (2µM) induced apoptosis in Jurkat cells at different times. The cells were collected and incubated with Annexin V-EGFP for 5 minutes according to the kit instructions. Analyze the results by flow cytometry.

All products and services are for Research Use Only. Do Not use in humans.

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