AMPK Phosphorylation Assay Kit (Fluorometric) (CAT#: CB-P012-K) Datasheet

Description
Creative Biolabs' cell-based ELISA measures the phosphorylated AMPK in whole cells and normalizes the signal to total protein content. The antibody can recognize two α subunits, so it can be used in cells of all tissues (human, mouse, rat). This simple and effective assay method eliminates the need for cell lysate preparation and can be used for AMPK regulation studies in short-term and long-term assays. In this assay, cells grown in 96-well plates are fixed and permeabilized in the wells. Fluorescence ELISA was used to measure AMPK phosphorylation (pAMPK), and then total protein was measured in each well.
Features
•Sensitive. Only 500 cells/well are needed to measure pAMPK modulation.
•Simple. Without cell lysis, cells can be cultured for several days.
Applications
For quantitative fluorescence immunoenzymatic determination of AMPK phosphorylation status in cultured cells.
Size
100 tests
Detection Method
FL360/450nm, 530/585nm
Sample
Cell, tissue etc
Species Reactivity
Human, mouse, rat
Protocol Length
8 hrs
Components
10x Wash Buffer: 25 mL Blocking Buffer: 25 mL Protein Stain: 6 mL HRP Substrate: 6 mL pAMPK-Ab1 10 uL HRP-Ab2 10 uL
Requires
37% formaldehyde, 3% H2O2, black (transparent bottom) cell culture 96-well plate, plate sealant: deionized water or distilled water; pipette; cell culture incubator; centrifuge tube can read ex / em = 530 / 585nm And ex/em=360/450nm fluorescent plate reader
Shipping
Gel Pack
Storage
Store all reagents at -20°C
Shelf Life
6 months
Research Use
For Research Use Only! Not For Use in Humans.

All products and services are for Research Use Only. Do Not use in humans.

ONLINE INQUIRY

Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

  • *
  • *
  • *
USA

UK