AMPK Phosphorylation Assay Kit (Fluorometric) (CAT#: CB-P012-K) Datasheet

Creative Biolabs' cell-based ELISA measures the phosphorylated AMPK in whole cells and normalizes the signal to total protein content. The antibody can recognize two α subunits, so it can be used in cells of all tissues (human, mouse, rat). This simple and effective assay method eliminates the need for cell lysate preparation and can be used for AMPK regulation studies in short-term and long-term assays. In this assay, cells grown in 96-well plates are fixed and permeabilized in the wells. Fluorescence ELISA was used to measure AMPK phosphorylation (pAMPK), and then total protein was measured in each well.

•Sensitive. Only 500 cells/well are needed to measure pAMPK modulation.
•Simple. Without cell lysis, cells can be cultured for several days.

For quantitative fluorescence immunoenzymatic determination of AMPK phosphorylation status in cultured cells.