Description
Creative Biolabs' total protein analysis kit is based on an improved pyrogallol red molybdate protein dye binding analysis. The color intensity at 600nm is directly proportional to the total protein concentration in the sample. This single reagent can be used to quantify total protein in urine (proteinuria) and cerebrospinal fluid (blood-brain barrier).
Features
• Fast and sensitive. The measurement requires 10 minutes. The linear detection range is 5-200 mg/dL protein.
• Robust and reliable. The substance in the urine interferes the least (<2%).
• Convenient and high throughput. Homogeneous "mixed cultivation measures" type determination. The HTS liquid handling system can be easily automated and can process thousands of samples per day.
Applications
For quantitative determination of total protein.
Size
100 tests
Detection Method
OD600nm (Pyrogallol Red)
Detection Limit
5 mg/dL
Sample
Urine, cerebrospinal fluid etc
Protocol Length
10 min
Components
Reagent 20 mL, Standard 1 mL 100 mg/dL
Requires
For 96-well plate determination: pipetting equipment, transparent flat-bottomed 96-well plate and plate reader.
For cuvette measurement: pipettes, cuvettes and spectrophotometers capable of reading OD600nm.
Shipping
Gel Pack
Storage
Store kit at 2-8°C.
Shelf Life
6 months
Research Use
For Research Use Only! Not For Use in Humans.
