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Phosphoglucomutase Activity Assay Kit (Colorimetric) (CAT#: CB-P172-K) Datasheet

Product Type
Kit
Description
In Creative Biolabs' phosphoglucose mutant enzyme activity assay kit (colorimetric), PGM (phosphoglucoamylase) can convert 1 phosphate glucose into 6 phosphate glucose. Glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH, thereby reducing the colorless probe to a colored product with strong absorption at 450 nm. The glucose phosphate mutant enzyme assay kit is simple, sensitive, and rapid, and can detect PGM activity even less than 1 mU/reaction.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Detection Limit
2.5 nmol/well - 12.5 nmol/well
Sensitivity
< 1 mU/well
Sample
Plasma, Tissue, Adherent cells, Suspension cells, Tissue Homogenate
Species Reactivity
Mammals
Protocol Length
0h 40m
Components
NADH Standard 1 vial
PGM Assay Buffer 1 x 27ml
PGM Developer 1 vial
PGM Enzyme Mix 1 vial
PGM Positive Control 1 vial
PGM Substrate 1 vial
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

All products and services are for Research Use Only. Do Not use in humans.

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