Phosphoglucomutase Activity Assay Kit (Colorimetric) (CAT#: CB-P172-K) Datasheet

Product Type
Kit
Description
In Creative Biolabs' phosphoglucose mutant enzyme activity assay kit (colorimetric), PGM (phosphoglucoamylase) can convert 1 phosphate glucose into 6 phosphate glucose. Glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH, thereby reducing the colorless probe to a colored product with strong absorption at 450 nm. The glucose phosphate mutant enzyme assay kit is simple, sensitive, and rapid, and can detect PGM activity even less than 1 mU/reaction.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Detection Limit
2.5 nmol/well - 12.5 nmol/well
Sensitivity
< 1 mU/well
Sample
Plasma, Tissue, Adherent cells, Suspension cells, Tissue Homogenate
Species Reactivity
Mammals
Protocol Length
0h 40m
Components
NADH Standard 1 vial
PGM Assay Buffer 1 x 27ml
PGM Developer 1 vial
PGM Enzyme Mix 1 vial
PGM Positive Control 1 vial
PGM Substrate 1 vial
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Standard curve with background signal subtracted (duplicates; +/- SD).

Figure 2 NADH standard curve produced using kit protocol.

Figure 3 Phosphoglucomutase activity in positive controls

All products and services are for Research Use Only. Do Not use in humans.

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