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MR Glycan Release Kit [For Glycan Release] (CAT#: CB-P273-K) Datasheet

Product Type
Kit
Description
The MR Glycan release kit includes a 96-well membrane bottom plate and other reagents for reduction, alkylation and immobilization of glycoproteins, sample cleanup and PNGase F glycan release (PNGaseF is provided separately).
Features
Number of Samples: Sufficient for up to 96 samples.
Amount of Sample: Up to 100 µg glycoprotein per well, dependent on sample.
Suitable Samples: Glycoproteins.
Applications
The Membrane Release (MR) kit is used for immobilizing, clean-up and PNGaseF mediated release of glycans from glycoproteins. The ability to immobilize glycoproteins allows the removal of low molecular weight solvent/buffer components, such as trehalose, which may interfere with glycan analysis.
Sample
Glycoproteins and glycopeptides containing N-linked glycans
Components
96 Glycan Release Membrane Plate 1+ Plate Lid 1
Iodoacetamide 19 mg dried 2
Dithiothreitol 77 mg dried 2
Block solution 6 mL liquid 1
Formic acid 100 µL liquid 2
20mM sodium bicarbonate 6 mL liquid 1
Handling Advice
1. Add up to 1 nmole of oligosaccharide to a tube.
2. Add de-ionized water to a total of 15 µL.
3. Add 4 µL of 5x Reaction Buffer 5.0.
4. Add 1 µL of α(1-3,4)-Fucosidase.
5. Incubate for 1 hour at 37°C.
Shipping
The product should be shipped at ambient temperature.
Storage
Storage conditions vary. Please check the individual kit components for their storage conditions.
Shelf Life
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Research Use
For research use only. Not for human or drug use

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All products and services are for Research Use Only. Do Not use in humans.

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