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LDH Cytotoxicity Fluorometric Assay Kit (CAT#: CB-P019-K) Datasheet

Description
Lactate dehydrogenase (LDH, EC 1.1.1.27) is a stable enzyme that exists in all cell types. Cell damage leads to the release of LDH enzyme into the culture medium, and its activity is widely used as a marker of cytotoxicity. It reversibly converts lactic acid to pyruvate, accompanied by the mutual conversion of NADH and NAD +. Creative Biolabs' LDH Cytotoxicity Fluorescence Assay Kit provides a sensitive, fast and simple method for detecting LDH released from damaged cells. In this assay, LDH converts lactic acid to pyruvate and NADH, thereby reducing the proprietary probe to a strong fluorescent product (Ex / Em = 535/587 nm). The amount of fluorescence is directly proportional to the number of damaged cells. The assay is suitable for high-throughput format and can be completed in less than 20 minutes.
Features
•Highly sensitive: up to 100 cells can be detected.
•Quick agreement: less than 20 minutes.
•High-throughput adaptability.
Applications
Determination of LDH released in the culture medium
Evaluation of the cytotoxicity of test compounds
Size
500 assays
Detection Method
Fluorescence (Ex/Em = 535/587 nm)
Sensitivity
~100 cells
Sample
Cell culture (adherent and non adherent)
Species Reactivity
Mammalian
Components
LDH Assay Buffer
LDH Substrate Mix
Cell Lysis Solution
LDH Positive Control
Shipping
Gel Pack
Storage
-20°C
Shelf Life
24 months from the date of purchase, if it is properly stored.
Research Use
For Research Use Only! Not For Use in Humans.

Figure: (a) Untreated Jurkat cells (low control) or treated with cell lysate for 30 minutes. (High control), (b) Relative fluorescence units of untreated Jurkat cells (low control) or treated with cell lysate for 30 minutes. (High control), (c) Treat HeLa cells overnight with 100 µM staurosporine or 3 µM cycloheximide. Measure the LDH released into the culture medium as well as the blank, untreated cells (low control), LDH positive control and lysed cells (high control). Perform the determination according to the kit protocol.


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All products and services are for Research Use Only. Do Not use in humans.

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