Description
Lactate dehydrogenase (LDH, EC 1.1.1.27) is a stable enzyme that exists in all cell types. Cell damage leads to the release of LDH enzyme into the culture medium, and its activity is widely used as a marker of cytotoxicity. It reversibly converts lactic acid to pyruvate, accompanied by the mutual conversion of NADH and NAD +. Creative Biolabs' LDH Cytotoxicity Fluorescence Assay Kit provides a sensitive, fast and simple method for detecting LDH released from damaged cells. In this assay, LDH converts lactic acid to pyruvate and NADH, thereby reducing the proprietary probe to a strong fluorescent product (Ex / Em = 535/587 nm). The amount of fluorescence is directly proportional to the number of damaged cells. The assay is suitable for high-throughput format and can be completed in less than 20 minutes.
Features
•Highly sensitive: up to 100 cells can be detected.
•Quick agreement: less than 20 minutes.
•High-throughput adaptability.
Applications
Determination of LDH released in the culture medium
Evaluation of the cytotoxicity of test compounds
Size
500 assays
Detection Method
Fluorescence (Ex/Em = 535/587 nm)
Sensitivity
~100 cells
Sample
Cell culture (adherent and non adherent)
Components
LDH Assay Buffer
LDH Substrate Mix
Cell Lysis Solution
LDH Positive Control
Shipping
Gel Pack
Storage
-20°C
Shelf Life
24 months from the date of purchase, if it is properly stored.
Research Use
For Research Use Only! Not For Use in Humans.
