LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (CAT#: CB-P144-K) Datasheet

Product Type
Kit
Description
Lactate dehydrogenase (LDH) analysis kit (colorimetric) can quantitatively detect LDH activity in various samples (such as serum or plasma, tissue, cells and culture medium).
In the LDH analysis protocol, LDH reduces NAD to NADH and then interacts with a specific probe to produce color (maximum OD = 450 nm). The kit can directly detect 1-100 mU/mL LDH in the sample. The assay is fast, convenient and sensitive.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Detection Limit
1 mU/ml - 100 mU/ml
Sensitivity
> 1 mU/ml
Sample
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
Species Reactivity
Mammals
Protocol Length
0h 30m
Components
LDH Assay Buffer 1 x 50ml
LDH Positive Control (lyophilized) 1 vial
LDH Substrate Mix 1 vial
NADH Standard 1 vial
Requires
Microplate reader
Handling Advice
LDH assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze every 2-3 min for at least 30 min with microplate reader in kinetic mode at 37°C
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Kinetic profiles of approx 0.5 mU of a sample of pure LDH (Positive Control) and 2 μL frozen human serum using buffer as a background control.


Figure 2 NADH Standard Curve.


Figure 3 Cellular necrosis was measured as LDH release after cisplatin treatment for 24 h and 48 h using ab102526. The release of LDH was apparently increased at 24 and 48 h after cisplatin treatment.

All products and services are for Research Use Only. Do Not use in humans.

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