Product Type
Kit
Description
The L-lactic acid assay kit (colorimetric) uses a measurement protocol in which lactic acid is oxidized by lactate dehydrogenase to produce a product that interacts with the probe to produce color (λmax = 450 nm).
The kit can detect L(+)-lactic acid in biological samples such as serum or plasma, cells, tissues, cell cultures and fermentation media.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric
Detection Limit
0.02 mM - 10 mM
Sensitivity
0.02 mM
Sample
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate
Protocol Length
0h 30m
Components
L(+)-Lactate Standard (100 nmol/µl) 1 x 100µl
Lactate Assay Buffer 1 x 25ml
Lactate Enzyme Mix (lyophilized) 1 vial
Lactate Substrate Mix 1 vial
Requires
Microplate reader
Handling Advice
Lactate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at room temp
- analyze with microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use
