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L-Lactate Assay Kit (Colorimetric) (CAT#: CB-P140-K) Datasheet

Product Type
Kit
Description
The L-lactic acid assay kit (colorimetric) uses a measurement protocol in which lactic acid is oxidized by lactate dehydrogenase to produce a product that interacts with the probe to produce color (λmax = 450 nm).
The kit can detect L(+)-lactic acid in biological samples such as serum or plasma, cells, tissues, cell cultures and fermentation media.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric
Detection Limit
0.02 mM - 10 mM
Sensitivity
0.02 mM
Sample
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate
Species Reactivity
Mammals
Protocol Length
0h 30m
Components
L(+)-Lactate Standard (100 nmol/µl) 1 x 100µl
Lactate Assay Buffer 1 x 25ml
Lactate Enzyme Mix (lyophilized) 1 vial
Lactate Substrate Mix 1 vial
Requires
Microplate reader
Handling Advice
Lactate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at room temp
- analyze with microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

All products and services are for Research Use Only. Do Not use in humans.

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