Glycerol Assay Kit (Fluorescent), Cell-Based (CAT#: CB-P164-K) Datasheet

Kit

The glycerol cell-based assay kit provides a simple, sensitive, and simple assay for determining the concentration of glycerol in various cell and tissue culture samples. The assay uses a glycerol-specific enzymatic reaction to convert the probe into a fluorescent product in the presence of glycerol. The glycerol level can be as low as 50 pmol per well, or 1.0 µM in the medium. Chloroquine (included in the kit as a control) causes steatosis and prevents autophagy, leading to accumulation of lipid droplets and an increase in free glycerol concentration.

Functional Studies