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Glycerol Assay Kit (Fluorescent), Cell-Based (CAT#: CB-P164-K) Datasheet

Product Type
Kit
Description
The glycerol cell-based assay kit provides a simple, sensitive, and simple assay for determining the concentration of glycerol in various cell and tissue culture samples. The assay uses a glycerol-specific enzymatic reaction to convert the probe into a fluorescent product in the presence of glycerol. The glycerol level can be as low as 50 pmol per well, or 1.0 µM in the medium. Chloroquine (included in the kit as a control) causes steatosis and prevents autophagy, leading to accumulation of lipid droplets and an increase in free glycerol concentration.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Sensitivity
1 µM
Sample
Cell culture media
Species Reactivity
Mammals
Protocol Length
2h 00m
Components
Glycerol Assay Buffer 1 x 1ml
Glycerol Probe (in DMSO) 1 x 10ml
Glycerol Enzyme Mix 1 vial
Free Glycerol Standard (100 mM) 1 vial
Chloroquine (5 mM) 1 x 17ml
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Glycerol Standard Curve.


Figure 2 Chloroquine Treatment.

All products and services are for Research Use Only. Do Not use in humans.

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