Glucose Assay Kit (Fluorometric), High Sensitivity (CAT#: CB-P151-K) Datasheet

Product Type
Kit
Description
The Picoprobe Glucose assay Kit is a simple, fast and ultra-sensitive for the assay of glucose. Suitable for high throughput use. In this assay, D-glucose is oxidized by the enzyme to form a product, which reacts with a colorless probe to produce Fluorometric (Ex / Em = 535/587 nm). The Fluorometric produced is proportional to the amount of glucose. The test kit can detect glucose less than 0.5 µM in various biological samples.
Applications
Functional Studies
Size
1 kit
Detection Method
Fluorometric
Sensitivity
< 0.5 µM
Sample
Serum, Plasma, Other biological fluids, Tissue, Adherent cells, Suspension cells, Tissue Culture Media
Species Reactivity
Mammals
Protocol Length
1h 00m
Components
Glucose Assay Buffer 1 x 25ml
Glucose Enzyme Mix (lyophilized) 1 vial
Glucose Standard 1 x 100µl
Glucose Substrate Mix 1 vial
PicoProbe 1 x 0.4ml
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Glucose standard curve


Figure 2 Measurement of glucose levels in samples
Measurement of glucose levels in human serum (1 µL of 1:10 diluted) and rat tissue lysates from liver, kidney and muscle (0.14 µg, 0.19 µg and 0.93 µg respectively).

All products and services are for Research Use Only. Do Not use in humans.

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