Glucose Assay Kit (Colorimetric/Fluorometric) (CAT#: CB-P148-K) Datasheet

Product Type
Kit
Description
The glucose assay kit is a fast, simple and sensitive for the quantitative assay of glucose levels in biological samples such as serum, plasma and other body fluids, food, and growth media.
In the glucose assay procedure, the glucose enzyme mixture oxidizes glucose to produce a product that reacts with the dye to produce color (λ = 570 nm) and Fluorometric (Ex / Em = 535/587 nm). The color and Fluorometric produced are directly proportional to the amount of glucose.
The kit can detect glucose in the range of 1-10000 µM.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Detection Limit
1 µM - 10000 µM
Sensitivity
1 µM
Sample
Tissue, Adherent cells, Suspension cells
Species Reactivity
Mammals
Protocol Length
0h 40m
Components
Assay Buffer 1 x 25ml
Glucose Enzyme Mix (lyophilized) 1 vial
Glucose Probe (in DMSO) 1 x 200µl
Glucose Standard (100 nmol/µl) 1 x 100µl
Requires
Microplate reader
Handling Advice
Glucose assay protocol summary:
- add samples (deproteinized) and standards to wells
- add reaction mix and incubate for 30 min at 37°C
- analyze with microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Standard curve: mean of duplicates (+/- SD) with background reads subtracted


Figure 2 Standard curve: mean of duplicates (+/- SD) with background reads subtracted


Figure 3 Glucose measured in cell lysates showing quantity (nmol) per million cells.


Figure 4 Glucose measured in human biological fluids showing quantity (µmol) per mL of tested sample. Samples were diluted 13.5 fold and measured colorimetrically.

All products and services are for Research Use Only. Do Not use in humans.

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