Product Type
Kit
Description
Enzymatic protein deglycosylation kits have been used for deglycosylation of various enzymes.
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Sample
Cell culture supernatant, Urine, Serum, Plasma, Tissue, Adherent cells, Suspension cells, Tissue Homogenate
Protocol Length
1h 00m
Components
PNGase F 1 mL/vial
O-Glycosidase 20 μL
α-2(3,6,8,9)-Neuraminidase 20 μL
Fetuin Control.5 mg
5× Reaction Buffer.2 mL
Denaturation Solution.1 mL
Triton™ X-100.1 mL
β-1→4-Galactosidase, positionally specific 20 μL
β-N-Acetylglucosaminidase 20 μL
Requires
Table-top shaker with heating unit.
Centrifuge suitable for 2 mL Eppendorf tubes (floor or table-top).
Centrifugal evaporator suitable for 2 mL Eppendorf tubes.
HPLC grade acetonitrile (1 mL). Do not use lower purity.
Acetic acid (1 mL).
Storage
2-8°C
Shelf Life
≥1 yr at 2‑8 °C
Research Use
For research use only. Not for human or drug use
