Enzymatic Antibody/Protein Deglycosylation Kit (CAT#: CB-P194-K) Datasheet

1 kit

Colorimetric/Fluorometric

Cell culture supernatant, Urine, Serum, Plasma, Tissue, Adherent cells, Suspension cells, Tissue Homogenate

Mammals

1h 00m

PNGase F 1 mL/vial
O-Glycosidase 20 μL
α-2(3,6,8,9)-Neuraminidase 20 μL
Fetuin Control.5 mg
5× Reaction Buffer.2 mL
Denaturation Solution.1 mL
Triton™ X-100.1 mL
β-1→4-Galactosidase, positionally specific 20 μL
β-N-Acetylglucosaminidase 20 μL

Table-top shaker with heating unit.
Centrifuge suitable for 2 mL Eppendorf tubes (floor or table-top).
Centrifugal evaporator suitable for 2 mL Eppendorf tubes.
HPLC grade acetonitrile (1 mL). Do not use lower purity.
Acetic acid (1 mL).

2-8°C

≥1 yr at 2‑8 °C

For research use only. Not for human or drug use