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Enzymatic Antibody/Protein Deglycosylation Kit (CAT#: CB-P194-K) Datasheet

Product Type
Kit
Description
Enzymatic protein deglycosylation kits have been used for deglycosylation of various enzymes.
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Sample
Cell culture supernatant, Urine, Serum, Plasma, Tissue, Adherent cells, Suspension cells, Tissue Homogenate
Species Reactivity
Mammals
Protocol Length
1h 00m
Components
PNGase F 1 mL/vial
O-Glycosidase 20 μL
α-2(3,6,8,9)-Neuraminidase 20 μL
Fetuin Control.5 mg
5× Reaction Buffer.2 mL
Denaturation Solution.1 mL
Triton™ X-100.1 mL
β-1→4-Galactosidase, positionally specific 20 μL
β-N-Acetylglucosaminidase 20 μL
Requires
Table-top shaker with heating unit.
Centrifuge suitable for 2 mL Eppendorf tubes (floor or table-top).
Centrifugal evaporator suitable for 2 mL Eppendorf tubes.
HPLC grade acetonitrile (1 mL). Do not use lower purity.
Acetic acid (1 mL).
Storage
2-8°C
Shelf Life
≥1 yr at 2‑8 °C
Research Use
For research use only. Not for human or drug use

All products and services are for Research Use Only. Do Not use in humans.

ONLINE INQUIRY

Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

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