D-Sorbitol Assay Kit (Colorimetric) (ab118968) (CAT#: CB-P178-K) Datasheet

Product Type
Kit
Description
Creative Biolabs' D-Sorbitol assay kit (colorimetric) is designed to measure sorbitol in various samples, such as food, fruit, juice, medicine, cosmetics, paper and some other biological samples. In the measurement, sorbitol is oxidized to fructose, which develops proportionally in a strong color with a maximum absorbance at 560 nm. This assay is useful in the range of 0.1-10 nmol sorbitol per sample.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Detection Limit
0.1 nmol/well - 10 nmol/well
Sensitivity
> 0.1 nmol/well
Sample
Tissue, Adherent cells, Suspension cells, Tissue Homogenate
Species Reactivity
Mammals
Protocol Length
0h 40m
Components
D-Sorbitol Assay Buffer 1 x 25ml
D-Sorbitol Colorimetric Probe 1 x 200µl
D-Sorbitol Developer (lyophilized) 1 vial
D-Sorbitol Enzyme Mix (lyophilized) 1 vial
D-Sorbitol Standard (100mM) 1 x 100µl
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 D-Sorbitol measured in various samples showing nmol/mg (fruits) and nmol/mL (juice). 300 mg (wet weight) of prune and pear was homogenised in 1 mL of water. Juice was diluted in water. Samples were diluted 2-1000 fold.

Figure 2 Standard curve: mean of duplicates (+/- SD) with background reads subtracted.

Figure 3 Functional Studies - D-Sorbitol Colorimetric Assay.

All products and services are for Research Use Only. Do Not use in humans.

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