Crystal Violet Cell Cytotoxicity Assay Kit (CAT#: CB-P016-K) Datasheet

Description
Crystal violet (CV) cytotoxicity assay is one of the commonly used methods for detecting cell viability or drug cytotoxicity. CV is a triarylmethane dye that can bind to ribose-type molecules (such as DNA in the nucleus). Normally, the dead adherent cells are detached from the cell culture plate and removed from the viable cell culture during the washing step. CV staining can be used to quantify the total DNA of the remaining population to determine cell viability. CV staining is directly proportional to cell biomass and can be measured at 570 nm. CV staining is a quick and universal method for screening cell viability under various stimulating or inhibiting conditions. Creative Biolabs' CV cell cytotoxicity analysis kit is simple, accurate, reproducible and sensitive. It also includes doxorubicin as a positive control. This kit provides an excellent and effective method for in vitro cytotoxicity research and high-throughput drug screening.
Features
The procedure is simple; less than 2 hours
Fast, sensitive and convenient
Applications
This assay provides a convenient and non-radioactive alternative to perform cytotoxicity assays. This assay relies on the ability of crystal violet to bind ribose molecules (ie DNA) in living cells.
Size
1000 assays
Detection Method
Detection method- Absorbance (570 nm)
Sample
Cell culture (adherent and non adherent)
Species Reactivity
Mammalian
Components
Crystal Violet Staining Solution
Shipping
Gel Pack
Storage
-20°C
Shelf Life
12 months
Research Use
For Research Use Only! Not For Use in Humans.

Figure: (a) 72-hour dose-response curves of HepG2 (b) and MCF-7 (b) cells to adriamycin determined by CV staining assay. The assay was performed in triplicate according to the kit protocol.

All products and services are for Research Use Only. Do Not use in humans.

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