IT Type
Antibody-based IT
Description
The recombinant anti-CD22 immunotoxin comprises a disulfide-linked Fv (VH/VL) anti-CD22 antibody fragment recombinantly connected to a 38 kD cytotoxic truncation of Pseudomonas exotoxin A ("PE", the 38 kD truncation is known in the art as "PE38"). PE38 is created by deleting from the 613 amino acid residue sequence of native PE residues 1-252, which correspond to domain I, along with residues 365-380 of domain Ib.
Indication
Hairy Cell Leukemia
Classification
Immuntoxin; biobetter
Construction
scFv-PE38
Synonyms
R490A
Cooperation Seeking
Creative Biolabs is interested in collaborating with potential partners (include but not limit to major pharma or biotech firms) to further co-develop our ADCC/CDC-enhanced antibodies or immutoxins. For commercial partners interested in our therapeutic biobetter product, Creative Biolabs welcomes collaboration.
Here are two ways for your choice, and please contact us for more details.
1) Collaborate with us and co-develop the programs from discovery phase to IND enabling. Costs will be shared.
2) Become a licensed candidate of our programs.
Looking forward to cooperating with you in the near future.
Official Name
CD22
Full Name
CD22 Molecule
Background
CD22 (CD22 Molecule) is a Protein Coding gene.
Diseases associated with CD22 include Refractory Hematologic Cancer and Refractory Hairy Cell Leukemia.
Among its related pathways are Hematopoietic Stem Cell Differentiation Pathways and Lineage-specific Markers and Innate Immune System.
Gene Ontology (GO) annotations related to this gene include carbohydrate binding.
An important paralog of this gene is SIGLEC1.
Alternative Names
CD22 Molecule; CD22 Antigen; Sialic Acid-Binding Ig-Like Lectin 2; B-Lymphocyte Cell Adhesion Molecule; T-Cell Surface Antigen Leu-14; SIGLEC-2;
Gene ID
Entrez Gene: 933
UniProt ID
UniProtKB: P20273
Antibody Clone
RFB4
Biologic Classification
Protein Based Therapies
Monoclonal antibody (mAb)
Antibody Type
scFv
Antibody Indication
Hairy Cell Leukemia
Toxic Moiety
PE38
Organism
Pseudomonas aeruginosa
Family
Pseudomonas exotoxin A
Molecular Mass
38 kDa
Toxic MOA
PE38 is a 38 kDa truncated form of Pseudomonas exotoxin (PE) containing amino acids 253–364 and 381–613. Pseudomonas exotoxin (or exotoxin A) is an exotoxin produced by Pseudomonas aeruginosa. Vibrio cholerae produces a similar protein called Cholix toxin (Q5EK40). It inhibits elongation factor 2. This is achieved by ADP ribosylation of EF2 using NAD +. This then causes the extension of the polypeptide to stop. This mechanism is similar to diphtheria toxin.
The antibody portion mediated selective binding to target cells, while the toxin portion mediated translocation into the cytosol and subsequent cell killing.
