C3a ELISA Kit [Human], 1 x 96 det. (CAT#: CB-P105-K) Datasheet

Product Type
Kit
Description
The activation products of the complement cascade contain new epitopes that are not present in various natural components. The human C3a ELISA kit is based on a captureable monoclonal antibody that can recognize a new epitope on C3a-desArg. This prevents cross-reaction with C3. "
The complement system is an important factor in innate immunity. The third complement component, C3, is essential to the classical, alternative and lectin pathways of complement activation. The synthesis of C3 is tissue-specific and is regulated in response to a variety of stimulants. In the process of complement activation, C3 is proteolytically cleaved, resulting in the release of the allergic peptide C3a.
C3a is a small polypeptide composed of 74 amino acids. C3a itself has a short lifespan, and it rapidly breaks down into more stable C3a-desArg (also known as acylation-stimulating protein, ASP) in the serum. Therefore, the measurement of C3a-desArg can draw reliable conclusions about the level of complement activation in the sample. For convenience, the following two forms will call it C3a.
C3a is a mediator of the local inflammatory process. It induces smooth muscle contraction, increases vascular permeability, and causes histamine to be released from mast cells and basophils. C3a is involved in the inflammatory response seen in Gram-negative bacterial sepsis, trauma, ischemic heart disease, post-dialysis syndrome and various autoimmune diseases. The normal value of plasma in the control group was between 48-150 ng/ml (median: 86.4 ng/ml, SD: 29.3 ng/ml).
The activation products of the complement cascade contain new epitopes that are not present in various natural components. The human C3a ELISA kit is based on a captureable monoclonal antibody that can recognize a new epitope on C3a-desArg. This prevents cross-reaction with C3.
Applications
The human C3a ELISA kit is used for the in vitro quantitative determination of human C3a / C3a-desArg in serum, plasma, bronchoalveolar lavage fluid (BALF) and urine samples.
Principle
Human C3a ELISA kit is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle. The working time is 3 hours. The effective format of two plates with twelve disposable 8-well strips allows free choice of batch size for determination. The samples and standards are incubated in microtiter wells coated with antibodies that recognize human C3a. The biotinylated tracer antibody will bind to the captured human C3a. The streptavidin-peroxidase conjugate will bind the biotinylated tracer antibody. The streptavidin-peroxidase conjugate will react with the substrate tetramethylbenzidine (TMB). The enzyme reaction is stopped by adding oxalic acid. Measure the absorbance at 450nm with a spectrophotometer. Draw a standard curve by plotting the absorbance (linear) relative to the corresponding concentration (logarithm) of the human C3a standard. The concentration of human C3a in a sample run at the same time as the standard can be determined from the standard curve.
Size
1 x 96 det.
Detection Method
Enzyme immunoassay (ELISA) technique
Detection Limit
31.3 to 2000 pg/ml
Sensitivity
31.3 pg/ml
Sample
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Specimen Collection
Blood samples were collected using sterile venipuncture techniques, and serum was obtained using standard procedures. At least 5 ml of whole blood is recommended. Let the blood clot in the serum test tube for 60-65 minutes at room temperature (20-25°C). Centrifuge the blood sample and transfer the cell-free serum to a clean test tube. The serum must be handled correctly to prevent complement activation in vitro. Serum should be frozen in a sealed tube at -70°C or lower for long-term storage or transport on dry ice. The sample should not be frozen and thawed more than once. Do not use jaundice, lipemia and hemolytic serum. You cannot use heat-inactivated serum. Plasma cannot be used.
Sample Volume
100 µl/well
Species Reactivity
Human
Protocol Length
2 hours
Storage
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.
Research Use
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.

All products and services are for Research Use Only. Do Not use in humans.

ONLINE INQUIRY

Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

  • *
  • *
  • *
USA

UK