Anti-NOTCH2 Antibody, Highly Galactosylated (CAT#: BioBet-GA-400ZP) Datasheet

NOTCH2

Notch 2

This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

Tarextumab; 1359940-55-8; OMP-59R5; 59M07; 13M57; anti-Notch2/3; OMP-59R5; NOTCH2; notch 2; Notch (Drosophila) homolog 2, Notch homolog 2 (Drosophila); neurogenic locus notch homolog protein 2; Notch homolog 2; hN2; AGS2; HJCYS;

Plasma membrane, Nucleus

Its related pathways are Neuroscience and Gene regulatory network modelling somitogenesis.

As receptors for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1), it regulates cell fate decisions. The released notch intracellular domain (NICD) activates the ligand, forms a transcription activator complex with RBPJ/RBPSUH, and activates the gene of the split site enhancer. Affect the implementation of differentiation, proliferation and apoptosis procedures. Participate in angiogenesis; negatively regulate endothelial cell proliferation, migration and angiogenesis budding. Participate in the maturation of CD4(+) and CD8(+) cells in the thymus. It is very important for follicle differentiation and cell fate selection in the follicle. During the development of the cerebellum, as the receptor of neuron DNER, it participates in the differentiation of Bergmann glial cells. Inhibit neuron and myogenic differentiation. May play an important role in post-planting development, possibly in some aspects of cell size and/or differentiation. May be involved in mesoderm development, somatic cell formation and neurogenesis. The function of HIF1A can be enhanced by isolating HIF1AN and HIF1A. THBS4 is necessary for the formation of protective astrocytes that regulate the subventricular zone (SVZ) niche after injury. Participate in the determination of left-right symmetry by adjusting the balance between the moving cilia of the left and right body (LRO) and the immobile (sensory) cilia.

Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody; Signaling Transduction antibody

Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by ADAM17 to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). Following endocytosis, this fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane. Phosphorylated. O-glycosylated on the EGF-like domains (PubMed:24226769). Contains both O-linked fucose and O-linked glucose in the EGF-like domains 11, 12 and 13, which are interacting with the residues on DLL4 (By similarity). O-linked glycosylation by GALNT11 is involved in determination of left/right symmetry: glycosylation promotes activation of NOTCH1, possibly by promoting cleavage by ADAM17, modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO) (PubMed:24226769). Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH. Monoubiquitination at Lys-1759 is required for activation by gamma-secretase cleavage, it promotes interaction with AAK1, which stabilizes it. Deubiquitination by EIF3F is necessary for nuclear import of activated Notch. Hydroxylated at Asn-1955 by HIF1AN. Hydroxylated at Asn-2022 by HIF1AN (By similarity). Hydroxylation reduces affinity for HI1AN and may thus indirectly modulate negative regulation of NICD (By similarity).

Protein Based Therapies
Monoclonal antibody (mAb)

IgG2, κ

BioBet-GA-400ZP

Human

Human

The Highly Galactosylated Anti-NOTCH2 (Tarextumab), a biobetter antibody contains with a high level of galactosylation.

Pancreatic cancers

Pancreatic cancers