Connect with Us at the Upcoming Events

With in-person events back again, Creative Biolabs is excited to greet you at the upcoming international conferences. Meet our team at 2022 BIO International Convention in June (booth #1754) and at the 13th Annual World ADC San Diego in September (booth number to be updated) for expert consultation on your drug discovery. Shoot an email to arrange an in-person meeting!

Book a Meeting

Anti-Mouse C3b/iC3b/C3c Monoclonal Antibody (clone 3/26) -0.5 mg (CAT#: CB-P394-AB) Datasheet

Product Type
Antibody
Description
Monoclonal antibody 3/26 recognizes the C3 fragments C3b, iC3b and C3c activated by mouse complement proteins. The complement system is an important factor in innate immunity. The third complement component, C3, is essential to the classical, alternative and lectin pathways of complement activation. C3 is the most abundant protein in the complement system, and the serum protein level is about 1.3 mg/ml. The proteolysis of certain enzymes causes the cleavage of C3 into C3a and C3b. C3b attaches to immune complexes and is further cleaved into iC3b, C3c, C3dg and C3f. The activation products of the complement cascade contain new epitopes that are not present in various natural components. The synthesis of C3 is tissue-specific and is regulated in response to a variety of stimulants. Hereditary C3 deficiency makes people vulnerable to frequent attacks from bacterial infections. In ulcerative colitis and idiopathic chronic inflammatory bowel disease, the deposition of C3 in the diseased mucosa has been reported. Monoclonal antibody 3/26 preferably recognizes cleaved C3 fragments C3b, iC3b and C3c. In the case of acute inflammation, many C3 are processed into products recognized by 3/26, so they can be used as markers of inflammation. Under chronic inflammatory conditions, the lowest reactivity with 3/26 can be observed. In this case, the C3dg product is mainly located in the inflammation (C3c is cleared) that the antibody (3/26) cannot recognize. The long-term processing/activation of C3 is at a low level, which will reduce the detection of C3 fragments C3b, iC3b and C3c.
Disease
Infectious diseases
Size
100 µg
Immunogen
The monoclonal antibody 3/26 recognizes mouse complement protein activated C3 fragments C3b, iC3b, and C3c. The complement system is an important factor in innate immunity. The third complement component, C3, is central to the classical, alternative and lectin pathways of complement activation. C3 is the most abundant protein of the complement system with serum protein levels of about 1.3 mg/ml. Proteolysis by certain enzymes results in the cleavage of C3 into C3a and C3b. C3b becomes attached to immune complexes and is further cleaved into iC3b, C3c, C3dg and C3f. Activation products of the complement cascade contain neo-epitopes that are not present in the individual native components. The synthesis of C3 is tissue-specific and is modulated in response to a variety of stimulatory agents. An inherited deficiency of C3 predisposes the person to frequent assaults of bacterial infections. In ulcerative colitis, and idiopathic chronic inflammatory bowel disease, the deposition of C3 in the diseased mucosa has been reported. The monoclonal antibody 3/26 preferably recognizes cleaved C3 fragments C3b, iC3b, and C3c. In case of an acute inflammatory reaction lots of C3 are processed into the products recognized by 3/26 and is as such useful as marker for inflammatory reaction. In chronic inflammatory conditions minimal reactivity with 3/26 may observed. In such cases primarily the C3dg product resides at the place of inflammation (C3c being cleared) which is not recognized by antibody 3/26. The chronic processing/activation of C3 is taking place at a lower level, which would reduce detection of the C3 fragments C3b, iC3b, and C3c.
Species
Mouse
Host
Rat
Clonality
Monoclonal
Isotype
Rat IgG2a
Clone Number
3/26
Buffer
PBS, containing 0.1% bovine serum albumin.
Application
Flow cytometry, Frozen sections, Functional studies, Immuno assays, Immuno fluorescence, Western blot
Application Notes
For immunohistochemistry, flow cytometry and western blotting, the diluent to be used depends on the detection system used. It is recommended that users test reagents and determine their own optimal dilution. The typical initial working dilution is 1:50. For functional studies, the in vitro dilution must be optimized in the user's experimental setting.
Storage Instructions
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Target
C3b/iC3b/C3c
Alternative Names
Complement C3; ASP; Plp; HSE-MSF; AI255234

All products and services are for Research Use Only. Do Not use in humans.

ONLINE INQUIRY

Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

  • *
  • *
  • *
USA

UK