Product Type
Antibody
Description
The polyclonal antibody recognizes human surfactant protein B (SP-B). There are four surfactant-specific proteins, which are called Surfactant Protein A (SP-A), SP-B, SP-C and SP-D. SP-A and SP-D are hydrophilic surfactant proteins and are members of the collectin family. SP-B and SP-C are hydrophobic surfactant proteins, which may be the most suitable indicators of the evolutionary origin of surfactants. SP-B is synthesized by alveolar type II epithelial cells into a 40-42 kD precursor, which is then processed by proteolysis to 7.8-8 kD. SP-B enhances the spreading and stability of surfactant phospholipids in alveoli. SP-B is essential for mammalian breathing, so it is largely protected. SP-B can interact with phospholipid head groups and fatty chains, and is particularly active in enhancing the surface activity of endogenous and exogenous lung surfactants. Even low SP-B content has a measurable effect in increasing dipalmitoylphosphatidylcholine (DPPC), mixed synthetic lipids and column-purified lung surfactant phospholipid adsorption, dynamic surface tension reduction and/or membrane resurfacing. The lack of SP-B and other surfactant components is related to respiratory distress syndrome (RDS) in premature infants and adults with respiratory distress syndrome (ARDS). To produce polyclonal antibodies against human-based 60 amino acid synthetic SP-B polypeptides. The antibody recognizes 42, 25 and 18 kD proteins under non-reducing conditions. In the presence of β-mercaptoethanol, the 18 kD form is reduced to 7.7-8 kD. This polyclonal antibody can cross-react with mouse, bovine and pig SP-B.
Disease
Pulmonology
Size
100 µg
Species
Human
Clonality
Polyclonal
Buffer
PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Application
Frozen sections, Immuno assays, Paraffin sections, Western blot
Application Notes
For immunohistology and western blotting, diluents will be used, depending on the detection system used. It is recommended that users test reagents and determine their own optimal dilution. The typical initial working dilution is 1:50.
Storage Instructions
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Alternative Names
SFTPB; previous SFTP3
