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Ficolin-1 ELISA kit [Human]-2 x 96 det. (CAT#: CB-P084-K) Datasheet

Product Type
Kit
Description
Ficolins are a type of pattern recognition molecules that contain both collagen-like domains and fibrinogen-like domains. They can activate the complement system on the surface of microorganisms by binding to MASP. In humans, three types of Ficolins are known: Ficolin-1 (M-ficolin), Ficolin-2 (L-ficolin) and Ficolin-3 (H-ficolin). Ficolin-1 is mainly expressed by monocytes, granulocytes and lung cells, and is locally present in the inflammation area. However, recent findings in serum suggest that Ficolin-1 also plays a role in systemic immunity like Ficolin-2 and Ficolin-3. The functional analysis of Ficolin-1 shows that it specifically binds to capsular polysaccharides of certain microorganisms. In addition, Ficolin-1 has been shown to promote the uptake of E. coli by leukemic monocytic lymphoma cell lines, indicating that it functions as a phagocytic receptor on the surface of circulating monocytes. The low concentration of Ficolin-1 in cord blood is associated with a higher mortality rate associated with necrotizing enterocolitis in premature infants, and the polymorphism of Ficolin-1 gene is related to the development of rheumatoid arthritis and susceptibility to leprosy.
Applications
Human Ficolin-1 ELISA kit is used for the in vitro quantitative determination of human Ficolin-1 in serum and plasma samples.
Principle
Human Ficolin-1 ELISA kit is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle. The working time is 3 hours. The effective form of the plate with twelve disposable 8-well plates allows free choice of batch size for determination. The samples and standards are incubated in microtiter wells coated with antibodies that recognize human Ficolin-1. The biotinylated tracer antibody will bind to the captured human M-ficolin. The streptavidin-peroxidase conjugate will bind the biotinylated tracer antibody. The streptavidin-peroxidase conjugate will react with the substrate tetramethylbenzidine (TMB). The enzyme reaction is stopped by adding oxalic acid. Measure the absorbance at 450nm with a spectrophotometer. Draw a standard curve by plotting the absorbance (linear) relative to the corresponding concentration (logarithm) of the human Ficolin-1 standard. The concentration of the human Ficolin-1 sample that runs simultaneously with the standard can be determined based on the standard curve.
Size
2 x 96 det.
Detection Method
Enzyme immunoassay (ELISA) technique
Detection Limit
3.1 to 200 ng/ml
Sensitivity
3.1 ng/ml
Sample
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Specimen Collection
Blood samples were collected using sterile venipuncture techniques, and serum was obtained using standard procedures. At least 5 ml of whole blood is recommended. Let the blood clot in the serum test tube for 60-65 minutes at room temperature (20-25°C). Centrifuge the blood sample and transfer the cell-free serum to a clean test tube. The serum must be handled correctly to prevent complement activation in vitro. Serum should be frozen in a sealed tube at -70°C or lower for long-term storage or transport on dry ice. The sample should not be frozen and thawed more than once. Do not use jaundice, lipemia and hemolytic serum. You cannot use heat-inactivated serum. Plasma cannot be used.
Sample Volume
100 µl/well
Species Reactivity
Human
Cross Reactivity
Bovine - No, Canine - No, Goat - No, Horse - No, Mouse - No, Pig - No, Rabbit - No, Rat - No, Sheep - No
Protocol Length
2 hours
Storage
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.
Research Use
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.

All products and services are for Research Use Only. Do Not use in humans.

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