Ficolin-1 ELISA kit [Human]-2 x 96 det. (CAT#: CB-P084-K) Datasheet

Kit

Ficolins are a type of pattern recognition molecules that contain both collagen-like domains and fibrinogen-like domains. They can activate the complement system on the surface of microorganisms by binding to MASP. In humans, three types of Ficolins are known: Ficolin-1 (M-ficolin), Ficolin-2 (L-ficolin) and Ficolin-3 (H-ficolin). Ficolin-1 is mainly expressed by monocytes, granulocytes and lung cells, and is locally present in the inflammation area. However, recent findings in serum suggest that Ficolin-1 also plays a role in systemic immunity like Ficolin-2 and Ficolin-3. The functional analysis of Ficolin-1 shows that it specifically binds to capsular polysaccharides of certain microorganisms. In addition, Ficolin-1 has been shown to promote the uptake of E. coli by leukemic monocytic lymphoma cell lines, indicating that it functions as a phagocytic receptor on the surface of circulating monocytes. The low concentration of Ficolin-1 in cord blood is associated with a higher mortality rate associated with necrotizing enterocolitis in premature infants, and the polymorphism of Ficolin-1 gene is related to the development of rheumatoid arthritis and susceptibility to leprosy.

Human Ficolin-1 ELISA kit is used for the in vitro quantitative determination of human Ficolin-1 in serum and plasma samples.

Human Ficolin-1 ELISA kit is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle. The working time is 3 hours. The effective form of the plate with twelve disposable 8-well plates allows free choice of batch size for determination. The samples and standards are incubated in microtiter wells coated with antibodies that recognize human Ficolin-1. The biotinylated tracer antibody will bind to the captured human M-ficolin. The streptavidin-peroxidase conjugate will bind the biotinylated tracer antibody. The streptavidin-peroxidase conjugate will react with the substrate tetramethylbenzidine (TMB). The enzyme reaction is stopped by adding oxalic acid. Measure the absorbance at 450nm with a spectrophotometer. Draw a standard curve by plotting the absorbance (linear) relative to the corresponding concentration (logarithm) of the human Ficolin-1 standard. The concentration of the human Ficolin-1 sample that runs simultaneously with the standard can be determined based on the standard curve.