Anti-Pyk-2 Antibody, Non-Fucosylated (BioBet-1463ZP) (CAT#: BioBet-1463ZP) Datasheet

PTK2B

protein tyrosine kinase 2 beta

This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

PTK2B; protein tyrosine kinase 2 beta; PKB; PTK; CAKB; FAK2; PYK2; CADTK; FADK2; RAFTK; protein-tyrosine kinase 2-beta; FADK 2; CAK-beta; protein kinase B; focal adhesion kinase 2; cell adhesion kinase beta; proline-rich tyrosine kinase 2; calcium-dependent tyrosine kinase; PTK2B protein tyrosine kinase 2 beta; related adhesion focal tyrosine kinase; calcium-regulated non-receptor proline-rich tyrosine kinase;

Plasma membrane, Nucleus

Diseases associated with PTK2B include Osteoporosis and Osteopetrosis.

Its related pathways are Translation Translation regulation by Alpha-1 adrenergic receptors and Sweet Taste Signaling.

Non-receptor protein tyrosine kinases regulate actin cytoskeleton reorganization, cell polarization, cell migration, adhesion, diffusion and bone remodeling. Participating in the regulation of humoral immune response is necessary for the normal level of splenic b-cells and the normal migration of splenic b-cells. This is necessary for the polarization and migration of normal macrophages to the site of inflammation. Regulate cytoskeleton rearrangement and cell proliferation in T cells, and participate in the regulation of T cell responses. Promote bone resorption by osteoclasts; this requires PTK2B/PYK2 and SRC. May inhibit the differentiation and activity of osteoprogenitor cells. It plays a role in downstream signal transduction of integrins, collagen receptors, immune receptors, g protein coupled receptors (GPCR), cytokines, chemokines and growth factor receptors, and mediates cellular stress responses. After activation, it forms a multi-subunit signaling complex with SRC and SRC family members; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulate many signal pathways. Promote the activation of phosphatidylinositol 3 kinase and AKT1 signaling cascade. Promote the activation of NOS3. Regulates the production of cell messenger cGMP. Promote the activation of MAPK1/ERK2 signal cascades such as MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promote the activation of Rho family GTPases, such as RHOA and RAC1. Recruit the ubiquitin ligase MDM2 and P53/TP53 in the nucleus to regulate P53/TP53 activity, P53/TP53 ubiquitination and proteasome degradation. As a scaffold, it binds to PDPK1 and SRC, allowing SRC to phosphorylate PDPK1 at'tyr9','tyr373' and'tyr376'. Promote the phosphorylation of NMDA receptors, members of the SRC family, thereby participating in the regulation of NMDA receptor ion channel activity and intracellular Ca(2+) levels. It is also possible to regulate potassium ion transport through phosphorylation of potassium channel subunits. Phosphorylate SRC; this increases the activity of SRC kinase. Phosphorylate ASAP1, NPHP1, KCNA2 and SHC1. Promote the phosphorylation of ASAP2, RHOU and PXN; this requires SRC and PTK2/PYK2.

Cancer antibody; Metabolism antibody; Signaling Transduction antibody

Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration; this activation is indirect and may be mediated by production of reactive oxygen species (ROS). Tyr-402 is the major autophosphorylation site, but other kinases can also phosphorylate Tyr-402. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-402 promotes interaction with SRC and SRC family members, leading to phosphorylation at Tyr-579; Tyr-580 and Tyr-881. Phosphorylation at Tyr-881 is important for interaction with GRB2. Phosphorylated on tyrosine residues upon activation of FGR and PKC. Recruitment by NPHP1 to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity. Phosphorylation by MYLK promotes ITGB2 activation and is thus essential to trigger neutrophil transmigration during lung injury. Dephosphorylated by PTPN12.

Protein Based Therapies
Monoclonal antibody (mAb)

IgG

BioBet-1463ZP

Rabbit

Human

This antibody recognizes Proline-rich/Ca2+-activated tyrosine kinase (Pyk2). The product can be used for glioblastoma multiforme.

Cancer

Cancer